Mechanism analysis for the differences in multi-level structure, enzyme accessibility and pasting properties of starch granules caused by different hydrolysis pathways of maltogenic α-amylase

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-01-04 DOI:10.1016/j.foodchem.2025.142789

Bo Zhang , Yuxiang Bai , Xiaoxiao Li , Jingjing Dong , Yanli Wang , Zhengyu Jin

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Abstract

The effect of pores distribution on the multi-scale structure, enzyme accessibility, and pasting properties of the waxy maize starch granules with the same degree of hydrolysis were examined. Increased maltogenic α-amylase (MA) dosage obviously increased the shallow pores number and the roughness, whereas extended time increased the holes depth. Despite achieving the same hydrolysis degree and specific surface area, samples with numerous shallow holes exhibited a higher mass fractal dimension, a lower, peak viscosity, final viscosity and setback. Besides, increased dosage prompted a sustained decrease in the number of short chains with DP 10–17; whereas prolonging time encouraged the continuous catalyzation in the same chains. Enzymatic probe profiles showed MA was more accessible to the amorphous region on the periphery of starch granules, rather than the inside. This finding provides a more valuable understanding of the catalytic mechanism for MA in heterogeneous systems and an accurate guidance for the industrial production.

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不同促麦芽α-淀粉酶水解途径导致淀粉颗粒多层次结构、酶可及性和糊化性能差异的机理分析

考察了孔隙分布对相同水解度的糯玉米淀粉颗粒的多尺度结构、酶可及性和糊化性能的影响。α-淀粉酶（MA）添加量的增加明显增加了浅孔数量和粗糙度，延长时间则增加了浅孔深度。尽管获得了相同的水解程度和比表面积，但具有许多浅孔的样品表现出更高的质量分形维数，更低的峰值粘度，最终粘度和挫折。此外，剂量增加导致DP 10-17短链数持续减少；而延长时间则有利于同一链上的连续催化。酶探针图谱显示，MA更容易进入淀粉颗粒外围的无定形区域，而不是内部。这一发现为多相体系中MA的催化机理提供了更有价值的认识，并为工业生产提供了准确的指导。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.