Portable Self-Powered/Colorimetric Dual-Mode Sensing Platform Based on Multifunctional Bioconjugates for Precise On-Site Detection of Acetamiprid

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-01-03 DOI:10.1021/acs.analchem.4c05692

Chengcheng Gu, Jianlin Liu, Ting Hou, Wenchao Zheng, Panpan Gai, Feng Li

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引用次数: 0

Abstract

Neonicotinoid insecticides have been widely applied in modern agriculture to improve crop productivity, but their residues have adverse impacts on the environment and human health. Hence, to address these issues, a portable self-powered/colorimetric dual-mode sensing platform was developed for the simple, rapid, precise, and sensitive on-site detection of acetamiprid (ATM) residues in vegetables. In this case, a multifunctional bioconjugate with specific recognition capability, excellent enzyme-like activity, and loading capacity is the key to the sensing design. Using an Eppendorf (EP) tube as a miniaturized laboratory, the bioconjugates and the colorimetric test strip were integrated into the EP tube, and combined with a nanozyme-based self-powered sensing platform (with an area of 1.5 × 1.5 cm²), accurate on-site detection of ATM was achieved, with detection limits as low as 2.9 fg·mL^–1 (self-powered method) and 31.5 fg·mL^–1 (colorimetry), respectively. Notably, we simulated the actual insecticidal situation based on the field application concentration and quantified the pesticide residues in vegetables. The relative errors of our method and the standard high-performance liquid chromatography method were 0.15% (the self-powered method) and 1.8% (colorimetry), respectively. The portable dual-mode sensing platform with high sensitivity and accuracy opened up a new avenue for the development of on-site pesticide residue analysis in the next generation of agricultural products.

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基于多功能生物偶联物的便携式自供电/比色双模传感平台对啶虫脒的精确现场检测

新烟碱类杀虫剂在现代农业中广泛应用，以提高作物生产力，但其残留对环境和人类健康造成了不利影响。为此，为了解决这些问题，研制了一种便携式自供电/比色双模传感平台，用于蔬菜中乙酰氨脒（ATM）残留的简单、快速、精确、灵敏的现场检测。在这种情况下，具有特定识别能力、优异的酶样活性和负载能力的多功能生物偶联物是传感设计的关键。采用epppendorf （epppendorf）试管作为小型实验室，将生物偶联物和比色试纸条集成到epppendorf试管中，结合基于纳米酶的自供电传感平台（面积为1.5 × 1.5 cm2），实现了ATM的精确现场检测，检测限分别低至2.9 fg·mL-1（自供电法）和31.5 fg·mL-1（比色法）。值得注意的是，我们根据现场施用浓度模拟了实际杀虫情况，量化了蔬菜中的农药残留。本方法与标准高效液相色谱法的相对误差分别为0.15%（自供电法）和1.8%（比色法）。高灵敏度、高精度的便携式双模传感平台，为下一代农产品现场农药残留分析的发展开辟了新的途径。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.