Preparation of peptides against immunocomplex of deltamethrin and application in noncompetitive lateral flow immunoassay

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-01-04 DOI:10.1016/j.foodchem.2025.142757

Panpan Cui , Jiao Li , Bingjie Chen , Zhiying Zhang , Yuan Ding , Hongwu Liang , Xiude Hua

{"title":"Preparation of peptides against immunocomplex of deltamethrin and application in noncompetitive lateral flow immunoassay","authors":"Panpan Cui , Jiao Li , Bingjie Chen , Zhiying Zhang , Yuan Ding , Hongwu Liang , Xiude Hua","doi":"10.1016/j.foodchem.2025.142757","DOIUrl":null,"url":null,"abstract":"<div><div>Lateral flow immunoassay (LFIA) has the advantages of simplicity and rapidness, and is widely used for the rapid detection of pesticides and other analytes. However, small molecule compounds such as pesticides are often analyzed using competitive LFIA (CLFIA), whose sensitivity often does not meet the actual needs. In this study, a noncompetitive LFIA (NLFIA) for deltamethrin (DM) with high sensitivity was developed by using anti-immunocomplex peptides (AIcPs). A total of 21 unique AIcPs were identified through the screening of phage-displayed cyclic peptide libraries. The AIcP containing the CYFDGAWYAC sequence in noncompetitive phage enzyme-linked immunosorbent assays (P-ELISA) exhibited a half signal saturation concentration (SC<sub>50</sub>) of 1.49 ng/mL, achieving a remarkable 209-fold sensitivity enhancement compared to antigen-based competitive ELISA. The AIcP, in the form of CYFDGAWYACGGGSSK-biotin, was synthesized to prepare AIcP-conjugated gold nanoparticles (AuNPs) by reacting with streptavidin (SA)-labeled AuNPs and subsequently assembled into the NLFIA strip. After optimization, the NLFIA achieved a visual limit of detection (vLOD) of 6.25 ng/mL in Tris-HCl buffer and 0.5 mg/kg in wheat, Chinese cabbage, and celery within 8 min. Furthermore, NLFIA detection results for blind wheat samples matched those of a reference liquid chromatography method.</div></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"471 ","pages":"Article 142757"},"PeriodicalIF":8.5000,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S030881462500007X","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Lateral flow immunoassay (LFIA) has the advantages of simplicity and rapidness, and is widely used for the rapid detection of pesticides and other analytes. However, small molecule compounds such as pesticides are often analyzed using competitive LFIA (CLFIA), whose sensitivity often does not meet the actual needs. In this study, a noncompetitive LFIA (NLFIA) for deltamethrin (DM) with high sensitivity was developed by using anti-immunocomplex peptides (AIcPs). A total of 21 unique AIcPs were identified through the screening of phage-displayed cyclic peptide libraries. The AIcP containing the CYFDGAWYAC sequence in noncompetitive phage enzyme-linked immunosorbent assays (P-ELISA) exhibited a half signal saturation concentration (SC₅₀) of 1.49 ng/mL, achieving a remarkable 209-fold sensitivity enhancement compared to antigen-based competitive ELISA. The AIcP, in the form of CYFDGAWYACGGGSSK-biotin, was synthesized to prepare AIcP-conjugated gold nanoparticles (AuNPs) by reacting with streptavidin (SA)-labeled AuNPs and subsequently assembled into the NLFIA strip. After optimization, the NLFIA achieved a visual limit of detection (vLOD) of 6.25 ng/mL in Tris-HCl buffer and 0.5 mg/kg in wheat, Chinese cabbage, and celery within 8 min. Furthermore, NLFIA detection results for blind wheat samples matched those of a reference liquid chromatography method.

查看原文本刊更多论文

抗溴氰菊酯免疫复合物肽的制备及其在非竞争横向流动免疫分析中的应用

横向流动免疫分析法（LFIA）具有简便、快速等优点，广泛应用于农药等分析物的快速检测。然而，对于农药等小分子化合物，通常采用竞争性LFIA （CLFIA）进行分析，其灵敏度往往不能满足实际需要。本研究利用抗免疫复合物肽（anti-immunocomplex peptides, AIcPs）构建了一种对溴氰菊酯（DM）具有高灵敏度的非竞争性LFIA （NLFIA）。通过筛选噬菌体环状肽文库，共鉴定出21个独特的aicp。在非竞争性噬菌体酶联免疫吸附试验（P-ELISA）中，含有CYFDGAWYAC序列的AIcP显示出半信号饱和浓度（SC50）为1.49 ng/mL，与基于抗原的竞争性ELISA相比，灵敏度提高了209倍。以cyfdgawyacgggssk -生物素的形式合成AIcP，与链亲和素（SA）标记的AuNPs反应制备AIcP共轭金纳米颗粒（AuNPs），随后组装到NLFIA条上。优化后，NLFIA在8 min内对Tris-HCl缓冲液的检出限为6.25 ng/mL，对小麦、大白菜和芹菜的检出限为0.5 mg/kg。此外，NLFIA对盲法小麦样品的检测结果与液相色谱法相吻合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.

文献相关原料

公司名称

产品信息

索莱宝

Streptavidin (SA)