Saccharomyces boulardii Ameliorates LPS-Induced Amyloidogenesis in Rats.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Probiotics and Antimicrobial Proteins Pub Date : 2024-12-30 DOI:10.1007/s12602-024-10445-7

Ghazaleh Mohammadi, Fatemeh Babaei, Faezeh Golpour, Fatemeh Sadat Rashidi, Shiva Ghafghazi, Leila Dargahi, Marjan Nassiri-Asl

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引用次数: 0

Abstract

Gut brain axis can affect the incidence of Alzheimer's disease (AD). Probiotics restore the homeostasis of gut dysbiosis and prevent AD. Here, we evaluated the impact of Saccharomyces boulardii on rats with lipopolysaccharide (LPS)-induced amyloidogenesis. Rats were classified into four groups: (1) Control (saline), (2) LPS 250 µg/kg (saline + LPS), (3) S. boulardii (10¹⁰ CFU/mL/rat), and (4) S. boulardii (10¹⁰ CFU/mL/rat) + LPS (250 μg/kg). The passive behavioral test, Western blotting, and immunohistochemistry were done using the animal hippocampi. Step-through latency (STL) indicated that the LPS-treated group had decreased memory retrieval compared to the control group. The LPS group had increased hippocampal levels of amyloid-β peptide, amyloid-β precursor protein (APP), and β-secretase (BACE). Administration of the S. boulardii before LPS prolonged STL which has been shortened in the LPS group (P < 0.05). In the LPS + S group, S. boulardii reduced the levels of APP significantly compared to the LPS group (P < 0.01). S. boulardii mitigated Aβ buildup and memory dysfunction caused by LPS through modulating the APP, BACE1, and Aβ pathways. Future studies are required to explain the neuroprotective effects of S. boulardii, since it could be a novel therapy or prevention strategy for AD.

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博氏酵母菌改善脂多糖诱导的大鼠淀粉样蛋白形成。

肠脑轴可影响阿尔茨海默病（AD）的发病率。益生菌恢复肠道生态失调的平衡，预防AD。在这里，我们评估了博拉氏酵母对脂多糖（LPS）诱导的淀粉样蛋白形成的大鼠的影响。将大鼠分为4组：(1)对照组（生理盐水），(2)LPS 250µg/kg（生理盐水+ LPS），(3)博氏弓形虫（1010 CFU/mL/大鼠），(4)博氏弓形虫（1010 CFU/mL/大鼠）+ LPS （250 μg/kg）。利用动物海马进行被动行为测试、免疫印迹和免疫组化。步过潜伏期（STL）显示lps治疗组与对照组相比记忆检索减少。LPS组海马淀粉样蛋白-β肽、淀粉样蛋白-β前体蛋白（APP）和β分泌酶（BACE）水平升高。LPS组STL缩短，LPS组STL延长(P

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.