A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

IF 6.3 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

NPJ Science of Food Pub Date : 2024-12-31 DOI:10.1038/s41538-024-00342-2

Saowalak Adunphatcharaphon, Oluwatobi Kolawole, Thanasat Sooksimuang, Waraporn Panchan, Wannee Wasuthep, Anuwut Petdum, Grit Pichayawaytin, Rungroj Jintamethasawat, Narusorn Doljirapisit, Armote Somboonkaew, Prae Noppakuadrittidej, Sudtida Kaew-amdee, Manlika Makornwattana, Julie Meneely, Christopher T. Elliott, Awanwee Petchkongkaew, Nitsara Karoonuthaisiri

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Abstract

Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins—aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.

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多重微阵列横向流动免疫分析装置同时测定水稻中5种真菌毒素。

由于多种真菌毒素对人类和动物的有害影响，它们的共存已成为全球日益关注的食品安全问题。本研究开发了一种环保的样品制备方法和一种创新的基于多重微阵列的横向流动免疫分析方法，使用一种新型便携式读取器现场同时检测水稻中5种受调控的真菌毒素——黄曲霉毒素B1、T-2毒素、玉米赤霉烯酮、脱氧雪腐菌烯醇和伏马毒素B1。生态友好、超快速的萃取过程采用生物基溶剂。主要是，通过一种新型发光有机染料产生的微阵列信号被捕获，使用安装有用户友好界面的便携式读取器来量化样品中的霉菌毒素水平。该方法可准确检测和定量这些真菌毒素，回收率为77%至127%。检出限范围为0.56 ~ 1.89 μg/kg，均低于法定检出限，相对标准偏差小于25%。该分析系统提供了一种现场检测水稻中多种真菌毒素的方法。

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来源期刊

NPJ Science of Food FOOD SCIENCE & TECHNOLOGY-

CiteScore

7.50

自引率

1.60%

发文量

期刊介绍： npj Science of Food is an online-only and open access journal publishes high-quality, high-impact papers related to food safety, security, integrated production, processing and packaging, the changes and interactions of food components, and the influence on health and wellness properties of food. The journal will support fundamental studies that advance the science of food beyond the classic focus on processing, thereby addressing basic inquiries around food from the public and industry. It will also support research that might result in innovation of technologies and products that are public-friendly while promoting the United Nations sustainable development goals.