Effects of Globularia cordifolia on colon cancer cells: antioxidant activity, inflammatory cytokine analysis and molecular docking studies

IF 3.3 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Emine Incilay Torunoglu, Erdi Can Aytar, Betul Aydın, Alper Durmaz, Özge Balpınar, Muhammed Sait Ertuğrul, Gül Fatma Yarım
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引用次数: 0

Abstract

BACKGROUND

The present study aimed to comprehensively evaluate the anticancer, anti-inflammatory, and antioxidant properties of Globularia cordifolia L. samples. The plant material was collected and extracted using the maceration method. Antioxidant activities were assessed through DPPH (i.e. 2,2-diphenyl-1-picrylhydrazyl) and iron chelation assays, whereas phenolic and flavonoid contents were measured using spectrophotometric methods. Gas chromatography-mass spectrometry analysis was performed to identify biologically active compounds. The cytotoxic effect on colon cancer cells was evaluated using MTT [i.e. 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] assays, and inflammatory cytokine levels were measured through an enzyme-linked immunosorbent assay. Molecular docking studies investigated the interactions of bioactive compounds with the 4UYA protein, and ADME (i.e. absorption, distribution, metabolism, excretion and toxicity) modeling predicted their toxicity.

RESULTS

The study revealed that the extract exhibited strong antioxidant activity with a median 50% inhibitory concentration (IC50) value of 39.48 ± 2.15 μg mL−1 in the DPPH assay and an effective iron chelation capacity with an IC50 value of 4.28 ± 0.19 mg mL−1. The total phenolic content was 662.51 ± 20.95 mg gallic acid equivalents g−1, and the flavonoid content was 114.99 ± 0.51 mg quercetin equivalents g−1, demonstrating the extract's richness in phenolic and flavonoid compounds. Significant antiproliferative effects were observed in colon cancer cells at a dose of 250 μg mL−1. Inflammatory cytokine analysis indicated a reduction in pro-inflammatory responses. Molecular docking studies showed strong binding affinities between the bioactive compounds and the 4UYA protein, suggesting the mechanisms of action.

CONCLUSION

The findings indicate that G. cordifolia extract exhibits strong antioxidant, anti-inflammatory and antiproliferative properties, making it a promising biomolecular agent for colon cancer therapy. The high phenolic and flavonoid contents, combined with the bioactive compounds' interactions with key molecular targets, underscore its potential therapeutic applications. © 2024 Society of Chemical Industry.

cordifolia对结肠癌细胞的影响:抗氧化活性、炎症细胞因子分析和分子对接研究。
背景:本研究旨在综合评价桔梗植物的抗癌、抗炎和抗氧化作用。样品采用浸渍法提取植物材料。通过DPPH(即2,2-二苯基-1-picrylhydrazyl)和铁螯合测定测定抗氧化活性,而用分光光度法测定酚类和类黄酮含量。采用气相色谱-质谱分析鉴定生物活性化合物。使用MTT[即2,3-二-(2-甲氧基-4-硝基-5-巯基)- 2h -四氮唑-5-羧基苯胺]测定法评估其对结肠癌细胞的细胞毒性作用,并通过酶联免疫吸附法测定炎症细胞因子水平。分子对接研究研究了生物活性化合物与4UYA蛋白的相互作用,ADME(即吸收、分布、代谢、排泄和毒性)模型预测了它们的毒性。结果:该提取物具有较强的抗氧化活性,DPPH试验中50%抑制浓度(IC50)中值为39.48±2.15 μg mL-1,有效的铁螯合能力为4.28±0.19 mg mL-1。总酚含量为662.51±20.95 mg没食子酸当量g-1,类黄酮含量为114.99±0.51 mg槲皮素当量g-1,表明提取物中酚类和类黄酮含量丰富。250 μg mL-1对结肠癌细胞有明显的抗增殖作用。炎症细胞因子分析表明,促炎反应减少。分子对接研究表明,活性化合物与4UYA蛋白具有较强的结合亲和力,提示其作用机制。结论:堇叶提取物具有较强的抗氧化、抗炎和抗增殖活性,是一种很有前景的生物分子药物。高酚类和类黄酮含量,结合生物活性化合物与关键分子靶点的相互作用,强调了其潜在的治疗应用。©2024化学工业学会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.10
自引率
4.90%
发文量
634
审稿时长
3.1 months
期刊介绍: The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.
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