A positively charged carbon dot complex improves the bioactivity of Isaria fumosorosea against Plutella xylostella (Linnaeus)

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2024-12-29 DOI:10.1186/s40538-024-00703-9

Xinyi Que, Fangmei Fu, Guorun Yang, Bingfu Lei, Shaukat Ali, Xingmin Wang

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引用次数: 0

Abstract

Background

Nanomaterials compounded with entomopathogenic fungi have been proven to be effective in insect pest management. This study reports the synthesis and bioactivity of Isaria fumosorosea-based nanoparticles by compounding the fungus with differentially (positively or negatively) charged carbon dots. Initially, negatively charged carbon dots (N-CDs) and carbon dots (P-CDs) were synthesized via a one-step hydrothermal method. The N-CDs and P-CDs were then individually compounded with I. fumosorosea to develop N-CD@I. fumosorosea and P-CD@I. fumosorosea nanocomposites.

Results

Characterization of the nanoparticles revealed that positively or negatively charged carbon dots were attached to I. fumosorosea by electrostatic bonding. Finally, the virulence of both types of nanoparticles was observed in Plutella xylostella. The bioassay results indicated that the highest P. xylostella mortality (92.7 ± 2.04%) was associated with the P-CD@I. fumosorosea treatment. The results of nontargeted metabolomic analysis revealed that different treatments affected the metabolism of P. xylostella by interfering with the riboflavin metabolism pathway by downregulating the expression of two main metabolites of the riboflavin metabolism pathway (2-5-diamino-6-pyrimidin-4-one and 7-hydroxy-6-methyl-8-ribiotin), although the extent of the expression downregulation varied among the different treatments.

Conclusion

Overall, this work provides insight into the mechanism by which positively charged nanomaterials improve the virulence of entomopathogenic fungi. This work provides a new direction for the design and application of nanomaterials for insect pest management.

Graphical Abstract

查看原文本刊更多论文

一种带正电的碳点配合物提高烟熏Isaria对小菜蛾的生物活性

纳米材料与昆虫病原真菌复合已被证明在害虫治理中是有效的。本研究报道了用不同（带正电或带负电）的碳点与烟沙菌复合制备的烟沙菌基纳米颗粒的合成及其生物活性。首先，通过一步水热法合成了带负电荷的碳点（N-CDs）和带负电荷的碳点（P-CDs）。然后将N-CDs和P-CDs分别与烟孢菌复配，得到N-CD@I。fumosorosea和P-CD@I。fumosorosea纳米复合材料。结果纳米颗粒的表征表明，带正电或负电的碳点可以通过静电键连接到褐藻上。最后，观察了两种纳米颗粒对小菜蛾的毒力。生物测定结果表明，与P-CD@I相关的小菜蛾死亡率最高（92.7±2.04%）。fumosorosea治疗。非靶向代谢组学分析结果显示，不同处理通过下调核黄素代谢途径的两种主要代谢物（2-5-二氨基-6-嘧啶-4- 1和7-羟基-6-甲基-8-核糖素）的表达来干扰小菜的代谢，但不同处理的表达下调程度不同。总之，本研究揭示了带正电的纳米材料提高昆虫病原真菌毒力的机制。本研究为纳米害虫防治材料的设计和应用提供了新的方向。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.