Vaccination with formulations targeting Eimeria maxima and Clostridium perfringens conferred comprehensive protection using a dual-infection challenge model of necrotic enteritis.

IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Qingzheng Zhang, Yue Yuan, Xianglin Pu, Lixin Xu, Xiaokai Song, Ruofeng Yan, Xiangrui Li, Charles Li, Cheng Yuan, Mingmin Lu
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引用次数: 0

Abstract

With increasing regulations restricting antibiotic use in animal feed, the need for alternative strategies to prevent and manage necrotic enteritis (NE) has become imperative. As a result, developing effective vaccines has emerged as a top priority for broiler chicken health management. Coccidial infections are a well-established predisposing factor for NE, underscoring the importance of controlling coccidiosis to help mitigate NE outbreaks. This research aimed to investigate the protective efficacy of vaccine preparations containing Eimeria maxima elongation factor-1α and a multicomponent antigen cocktail of Clostridium perfringens, including a single collagen adhesion protein (CpCna) and two chimeric proteins: CpNA (NetB-Alpha-toxin) and CpFZ (Fructose-1,6-bisphosphate aldolase-Zinc metalloprotease). Two vaccine preparations-recombinant subunit vaccines and DNA vaccines-were developed to assess their immunoprotective effects, determined by relative body weight gain rate, lesion scores, survival rates, and antigen-specific IgY levels using a dual-infection NE challenge model involving E. maxima and C. perfringens. Broilers were administered two subcutaneous immunizations with either adjuvanted proteins or eukaryotic expression plasmids on Days 7 and 17. Chickens vaccinated with the five antigens exhibited significantly higher serum antigen-specific IgY levels, improved weight gains, zero mortality, and reduced lesion scores following the lethal dual-infection challenge. These results indicated that vaccine preparations targeting both C. perfringens and E. maxima represent a promising approach for controlling and preventing coccidiosis-induced NE in chickens.

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来源期刊
Poultry Science
Poultry Science 农林科学-奶制品与动物科学
CiteScore
7.60
自引率
15.90%
发文量
0
审稿时长
94 days
期刊介绍: First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
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