Structure of Branched Subunits in the Amylopectins of Starches Varying in Polymorphism

IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY

ACS food science & technology Pub Date : 2024-11-21 DOI:10.1021/acsfoodscitech.4c0061210.1021/acsfoodscitech.4c00612

Chuanjie Chen, and , Fan Zhu*,

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Abstract

Amylopectins from model starches of different polymorphisms (A-type maize, C_A-type lotus seed, and B-type potato) were characterized with regard to the molecular structure of their branched subunits (clusters and building blocks). Lotus seed and maize clusters had larger sizes, shorter average chain lengths, higher branching densities, and shorter interblock chain lengths than potato clusters. Lotus seed and maize clusters were different in their composition of short internal chains. Lotus seed clusters consisted of fewer singly branched blocks and more doubly branched blocks than maize clusters. The clusters isolated from the two lotus seed amylopectins had similar chain length distribution but different building block compositions. The interbranch chain length was considered important in shaping starch polymorphism. Starch polymorphism might be related not only to the densely branched subunits of amylopectin but also to the loosely branched ones. In addition, this study exemplified a light-scattering-based method as an alternative for the size characterization of amylopectin branched subunits.

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淀粉支链蛋白分支亚基结构的多态性变化

对来自不同多态性模型淀粉（A 型玉米、CA 型莲子和 B 型马铃薯）的直链淀粉的支链亚基（簇和构件）分子结构进行了表征。与马铃薯簇相比，莲子和玉米簇具有更大的尺寸、更短的平均链长、更高的分支密度和更短的块间链长。莲子和玉米簇在内部短链的组成上有所不同。与玉米簇相比，莲子簇由较少的单支链块和较多的双支链块组成。从两种莲子直链淀粉中分离出的淀粉簇具有相似的链长分布，但结构单元组成不同。支链间长度被认为是形成淀粉多态性的重要因素。淀粉多态性可能不仅与支链密集的直链淀粉亚基有关，还与支链松散的亚基有关。此外，本研究还示范了一种基于光散射的方法，作为支链亚基大小表征的替代方法。

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来源期刊

ACS food science & technology

CiteScore

3.30

自引率

0.00%

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