The Protective Effects of Microbe Derived Antioxidants on Digestive Tissue Morphology, Functions, and Intestinal Microbiota Diversity of Eriocheir sinensis Exposed to Glyphosate

IF 3 2区农林科学 Q1 FISHERIES

Aquaculture Nutrition Pub Date : 2024-12-10 DOI:10.1155/anu/2620217

Yameng Song, Mengyao Wu, Yongxu Cheng, Chao Niu, Xiaowen Yu, Yangyang Pang, Xiaozhen Yang

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Abstract

Introduction: The use of glyphosate (Gly) has caused unnecessary economic losses to the aquaculture industry, but research on the effect of Gly on Eriocheir sinensis is very limited. The aim of this study is to reduce the negative effects of Gly, reduce yield loss, and improve economic benefits through nutritional feed control technology.

Methods: The experiment involved 80 crabs randomly divided into four groups: control group, Gly group (48.945 mg/L), microbe-derived antioxidant (MA) group, and Gly and MA treatment group. The study lasted for 7 days. In this study, the effects of Gly on the digestive function of E. sinensis were investigated using histology and spectrophotometer, and the gut microorganisms of E. sinensis were analyzed using high-throughput sequencing technology.

Results: The study found that exposure to Gly resulted in separation of the folds of the midgut mucosa of Eriocheir sinensis from the basement membrane, a decrease in the fold area of the hindgut mucosa, and an increase in the number of B cells in hepatic tubules. Additionally, the lipase activity of the intestine in the Gly group was significantly higher than that of the control group and the MA + Gly group, while the hepatopancreatic lipase decreased significantly. The amylase activity in the intestine and hepatopancreas of the Gly group was significantly lower than that of the control group. The trypsin activity in the hepatopancreas of the MA + Gly group was significantly higher than that of the Gly group. The Shannon diversity index in MA + Gly group was significantly lower than that in control group. At the phylum level, the abundance of the Campilobacterota in the MA + Gly group decreased. At the genus level, the proportion of the Citrobacter and Flavobacterium in the MA + Gly group decreased.

Conclusion: Gly has certain effects on the digestive tissue function, intestinal microbial diversity index and intestinal microbiota structure of E. sinensis, and MA can ameliorate the negative effects of Gly on E. sinensis.

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微生物源抗氧化剂对草甘膦暴露的中华绒螯蟹消化组织形态、功能和肠道微生物群多样性的保护作用

草甘膦（glyphosate, Gly）的使用给水产养殖业造成了不必要的经济损失，但关于草甘膦对中华绒螯蟹影响的研究非常有限。本研究旨在通过营养饲料控制技术，减少甘氨酸的负面影响，减少产量损失，提高经济效益。方法：将80只大闸蟹随机分为4组：对照组、甘氨酸组（浓度为48.945 mg/L）、微生物源抗氧化剂组、甘氨酸和甘氨酸处理组。试验期7 d。本研究采用组织学和分光光度计法研究了Gly对中华梭菌消化功能的影响，并采用高通量测序技术对中华梭菌肠道微生物进行了分析。结果：研究发现，Gly暴露导致中华绒螯蟹中肠粘膜褶皱与基膜分离，后肠粘膜褶皱面积减少，肝小管B细胞数量增加。Gly组肠道脂肪酶活性显著高于对照组和MA + Gly组，肝胰脂肪酶活性显著降低。Gly组肠道和肝胰腺淀粉酶活性显著低于对照组。MA + Gly组肝胰腺胰蛋白酶活性显著高于Gly组。MA + Gly组Shannon多样性指数显著低于对照组。在门水平上，MA + Gly组Campilobacterota丰度降低。在属水平上，MA + Gly组柠檬酸杆菌（Citrobacter）和黄杆菌（Flavobacterium）的比例下降。结论：Gly对中华绒螯蟹的消化组织功能、肠道微生物多样性指数和肠道菌群结构有一定影响，MA可改善Gly对中华绒螯蟹的负面影响。

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来源期刊

Aquaculture Nutrition 农林科学-渔业

CiteScore

7.20

自引率

8.60%

发文量

131

审稿时长

3 months

期刊介绍： Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers. Aquaculture Nutrition publishes papers which strive to: increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research. improve understanding of the relationships between nutrition and the environmental impact of aquaculture. increase understanding of the relationships between nutrition and processing, product quality, and the consumer. help aquaculturalists improve their management and understanding of the complex discipline of nutrition. help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.