Context-Dependent Estrogenic Actions of (+)-Pisatin Produced in Elicited Green or Snow Pea (Pisum sativum)

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Jorge A. Belgodere, Megan C. Benz, G. Wills Kpeli, Jack R. Elliott, Steven Elliott, Jack D. North, Isaac J. Ponder, Peng Ma, Sophie R. Dietrich, Thomas Cheng, Khoa Nguyen, Syreeta L. Tilghman, John A. McLachlan, Binghao Zou, Muralidharan Anbalagan, Brian Rowan, Mark Mondrinos, Thomas E. Wiese, Van T. Hoang, Bridgette M. Collins-Burow, Elizabeth C. Martin, Matthew E. Burow, Stephen M. Boué
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引用次数: 0

Abstract

Legumes are a predominant source of isoflavones, termed phytoestrogens, that mimic 17β-estradiol (E2). Phytoalexins are inducible isoflavones produced in plants subjected to environmental stressors (e.g., UV, heat, or fungi). This study investigated estrogenic activity of snow and green peas elicited with Aspergillus sojae. Elicited extracts increased estrogenic activity and proliferation of breast cancer cells (MCF-7 or T47D) in a dose-dependent manner but exhibited antiestrogenic activity when combined with synthetic E2. HPLC analysis of elicited pea extracts identified (+)-pisatin as the primary phytoalexin, which was produced significantly (p < 0.0001) more in snow pea compared to green pea. RNA sequencing results suggested potential functional effects on endothelial cells and tissue vascularization. Indeed, (+)-pisatin enhanced metrics of network assembly and maturation in a microphysiological model of bulk tissue vasculogenesis. Thus, context-dependent functional effects of (+)-pisatin and pharmacologically similar phytoestrogens on the entire tissue microenvironment should be considered in preclinical investigation as potential therapeutic agents.

Abstract Image

诱导绿豌豆或雪豌豆(Pisum sativum)产生的(+)-Pisatin的环境依赖性雌激素作用
豆类是异黄酮的主要来源,称为植物雌激素,类似于17β-雌二醇(E2)。植物抗毒素是植物受到环境胁迫(如紫外线、热或真菌)时产生的诱导异黄酮。研究了大豆曲霉诱导的雪豌豆和青豆的雌激素活性。诱导提取物以剂量依赖的方式增加雌激素活性和乳腺癌细胞(MCF-7或T47D)的增殖,但与合成E2联合使用时表现出抗雌激素活性。高效液相色谱分析表明(+)-pisatin是主要的植物抗毒素,其产量显著(p <;0.0001)比绿豌豆多。RNA测序结果提示对内皮细胞和组织血管化的潜在功能影响。事实上,(+)-pisatin在大组织血管发生的微生理模型中增强了网络组装和成熟的指标。因此,在临床前研究中,(+)-pisatin和药理学上类似的植物雌激素对整个组织微环境的环境依赖性功能效应应被视为潜在的治疗药物。
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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