Jorge A. Belgodere, Megan C. Benz, G. Wills Kpeli, Jack R. Elliott, Steven Elliott, Jack D. North, Isaac J. Ponder, Peng Ma, Sophie R. Dietrich, Thomas Cheng, Khoa Nguyen, Syreeta L. Tilghman, John A. McLachlan, Binghao Zou, Muralidharan Anbalagan, Brian Rowan, Mark Mondrinos, Thomas E. Wiese, Van T. Hoang, Bridgette M. Collins-Burow, Elizabeth C. Martin, Matthew E. Burow, Stephen M. Boué
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引用次数: 0
Abstract
Legumes are a predominant source of isoflavones, termed phytoestrogens, that mimic 17β-estradiol (E2). Phytoalexins are inducible isoflavones produced in plants subjected to environmental stressors (e.g., UV, heat, or fungi). This study investigated estrogenic activity of snow and green peas elicited with Aspergillus sojae. Elicited extracts increased estrogenic activity and proliferation of breast cancer cells (MCF-7 or T47D) in a dose-dependent manner but exhibited antiestrogenic activity when combined with synthetic E2. HPLC analysis of elicited pea extracts identified (+)-pisatin as the primary phytoalexin, which was produced significantly (p < 0.0001) more in snow pea compared to green pea. RNA sequencing results suggested potential functional effects on endothelial cells and tissue vascularization. Indeed, (+)-pisatin enhanced metrics of network assembly and maturation in a microphysiological model of bulk tissue vasculogenesis. Thus, context-dependent functional effects of (+)-pisatin and pharmacologically similar phytoestrogens on the entire tissue microenvironment should be considered in preclinical investigation as potential therapeutic agents.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.