Obesity phenotype and gut microbiota alterations are not associated with anxiety-like behaviour in high-fat diet-fed mice†

IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Food & Function Pub Date : 2024-12-10 DOI:10.1039/D4FO04461D
Giselle C. Wong, Bertrand Bearzatto, Jean-Luc Gala, Nathalie M. Delzenne, Matthias Van Hul and Patrice D. Cani
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Abstract

Anxiety is a common co-morbidity with obesity and metabolic disease, and can lead to a significant impact on quality of life. The vast differences in the gut microbiota between obese and control individuals provide a potential avenue for therapeutic intervention. A high-fat diet (HFD) in rodent models have been shown to induce anxiety-like behaviour and has been tested through an array of distinct behavioural tests such as the elevated plus maze test, light–dark test and open field test. Despite differences in testing and assessment parameters, the behavioural outcomes have previously yielded similar results. Recent evidence suggests that HFD has an anxiolytic effect on mice, complicating the model. Here, we aimed to confirm whether HFD-fed mice are more susceptible to presenting anxiety-like behaviours. Our findings showed no significant differences in behaviour, plasma corticosterone and inflammation markers between HFD and control diet (CTD) mice, despite considerable differences in adiposity and faecal microbial communities. Additionally, daily oral gavage is one of the most common methods for testing bacterial probiotics in rodent models, but this handling could potentially also cause stress to the mice. Thus, we investigated if daily oral gavage could mask differences in HFD and CTD mice. We found no significant differences in weight, fat mass or anxiety-like behaviour in CTD-fed mice with or without daily oral gavage.

Abstract Image

在高脂肪饮食喂养的小鼠中,肥胖表型和肠道微生物群的改变与焦虑样行为无关。
焦虑是肥胖和代谢性疾病的常见合并症,可对生活质量产生重大影响。肥胖者和对照组之间肠道微生物群的巨大差异为治疗干预提供了潜在的途径。啮齿类动物模型中的高脂肪饮食(HFD)已被证明会诱发类似焦虑的行为,并已通过一系列不同的行为测试进行了测试,如升高加迷宫测试、光暗测试和开阔场地测试。尽管在测试和评估参数方面存在差异,但行为结果以前产生了类似的结果。最近的证据表明,HFD对小鼠有抗焦虑作用,使模型复杂化。在这里,我们的目的是确认喂食hfd的小鼠是否更容易表现出焦虑样行为。我们的研究结果显示,HFD和对照饮食(CTD)小鼠在行为、血浆皮质酮和炎症标志物方面没有显著差异,尽管肥胖和粪便微生物群落存在相当大的差异。此外,每天口服灌胃是在啮齿动物模型中测试细菌益生菌的最常用方法之一,但这种处理也可能对小鼠造成压力。因此,我们研究了每日灌胃是否可以掩盖HFD和CTD小鼠的差异。我们发现,每天口服或不口服ctd喂养的小鼠在体重、脂肪量或焦虑样行为方面没有显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Food & Function
Food & Function BIOCHEMISTRY & MOLECULAR BIOLOGY-FOOD SCIENCE & TECHNOLOGY
CiteScore
10.10
自引率
6.60%
发文量
957
审稿时长
1.8 months
期刊介绍: Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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