Carob (Ceratonia siliqua) leaves: A comprehensive analysis of bioactive profile and health-promoting potential of an untapped resource

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-12-09 DOI:10.1016/j.foodchem.2024.142392

Simona Serio , Valentina Santoro , Rita Celano , Donatella Fiore , Maria Chiara Proto , Filomena Corbo , Maria Lisa Clodoveo , Roberta Tardugno , Anna Lisa Piccinelli , Luca Rastrelli

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引用次数: 0

Abstract

Carob is a resilient plant with ecological and nutritional significance. A comprehensive study of Ceratonia siliqua leaves (CSL) was conducted to explore its chemical composition and health-promoting potential, aiming at improving the carob-crop sustainability through valorization of this untapped co-product. UHPLC-DAD-HRMS/MS quali-quantitative profiling of CSL revealed high levels of n-galloylated glucoses (129–196 mg g⁻¹) and flavonol-glycosides (36–42 mg g⁻¹), with siliquapyranone (39–56 mg g⁻¹), 1,2,3,6-tetragalloylglusose (47–69 mg g⁻¹) and myricitrin (27–33 mg g⁻¹) as main markers. Highest bioactive content and antioxidant capacity (5.0 and 3.1 TEAC) was obtained during pods harvesting. Besides significant antioxidant potential, CSL exhibited strong-high inhibitory activity against α-glucosidase, acetylcholinesterase and butyrylcholinesterase (IC₅₀, 0.51, 13.5 and 58.0 μg mL⁻¹, respectively). 1,2,3,6-tetragalloylglusose and siliquapyranone are the main contributors to antioxidant and α-glucosidase inhibitory capacities. Excellent ability of extract and 1,2,3,6-tetragalloylglusose (IC₅₀, 0.05 μM) in selectively inhibiting α-glucosidase, make them promising candidates to manage hyperglycemia with fewer side effects.

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角豆（Ceratonia silqua）叶：一种未开发资源的生物活性特征和促进健康潜力的综合分析

角豆是一种具有生态和营养意义的抗逆性植物。摘要对角豆树叶片（Ceratonia silqua leaves， CSL）的化学成分及其促进健康的潜力进行了全面研究，旨在通过对这一尚未开发的副产品进行价值增值，提高角豆树作物的可持续性。UHPLC-DAD-HRMS / MS quali-quantitative CSL的分析显示高水平的n-galloylated葡萄糖生成(129 - 196年 毫克 克−1)和flavonol-glycosides(36-42 毫克 克−1),与siliquapyranone(39-56 毫克 克−1),1,2,3,6-tetragalloylglusose(47 - 69 毫克 克−1)和myricitrin(即 毫克 克−1)为主要标志。收获豆荚时，其生物活性含量和抗氧化能力最高，分别为5.0和3.1 TEAC。除显著的抗氧化能力外，CSL对α-葡萄糖苷酶、乙酰胆碱酯酶和丁基胆碱酯酶均表现出较高的抑制活性（IC50、0.51、13.5和58.0 μg mL−1）。1,2,3,6-四烯丙基葡萄糖和硅吡喃酮是抗氧化能力和α-葡萄糖苷酶抑制能力的主要贡献者。提取物和1,2,3,6-四烯丙基葡萄糖（IC50, 0.05 μM）选择性抑制α-葡萄糖苷酶的能力优异，是治疗高血糖的有希望的候选药物，而且副作用小。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.