Rutin Attenuates the Oxidative Damage Induced by Zearalenone in Piglet Endometrial Stromal Cells via the p53 Signaling Pathway

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-06 DOI:10.1021/acs.jafc.4c09143

Chenlong Wang, Mengya Wang, Yueru Yang, Chuangjiang Chen, Sajid Ur Rahman, Zhiping Yang, Hongyan Ding, Wanyue Huang, Xichun Wang

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Abstract

Zearalenone (ZEA) induces oxidative damage in porcine endometrial stromal cells (ESCs), which is a critical factor affecting the growth and reproduction of female pigs. We hypothesize that rutin, a flavonoid antioxidant, can alleviate ZEA-induced cellular damage through the p53 signaling pathway. In this experiment, porcine ESCs were used as a research model. After transfection with siRNA to silence the p53 gene, rutin and ZEA were added to the cocultured porcine ESCs. Rutin notably reduced the ZEA-induced apoptosis rate, ROS, MDA, and 4-HNE levels via the p53 pathway; lowered the expression of LC3, Beclin-1, p62, Bax, and CHOP; and significantly improved cell viability, GSH-Px, T-SOD enzyme activity, and Bcl-2 expression. It also inhibited both apoptosis and autophagy. These findings suggested a novel antioxidant mechanism by which rutin mitigated the toxic effects of ZEA, highlighting the role of the p53 gene knockdown in this process.

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芦丁通过p53信号通路减弱玉米赤霉烯酮对仔猪子宫内膜间质细胞的氧化损伤

玉米赤霉烯酮（ZEA）可诱导猪子宫内膜基质细胞（ESCs）氧化损伤，是影响母猪生长和繁殖的重要因素。我们推测，黄酮类抗氧化剂芦丁可以通过p53信号通路减轻zea诱导的细胞损伤。本实验以猪ESCs为研究模型。转染siRNA沉默p53基因后，加入芦丁和ZEA共培养猪ESCs。芦丁通过p53通路显著降低zea诱导的细胞凋亡率、ROS、MDA和4-HNE水平；LC3、Beclin-1、p62、Bax、CHOP表达降低；显著提高细胞活力、GSH-Px、T-SOD酶活性和Bcl-2表达。它还能抑制细胞凋亡和自噬。这些发现提示了一种新的抗氧化机制，芦丁可以减轻ZEA的毒性作用，并强调了p53基因敲低在这一过程中的作用。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.