hsa_circ_0000520 Serves as a Prognostic Biomarker for Colorectal Cancer and Promotes in the Disease Progression.

Abstract

Background/aims: Colorectal cancer (CRC) constitutes one of the prevalent malignancies within the gastrointestinal tract and serves as a primary contributor to cancer-related mortalities. This investigation sought to investigate the expression and prognostic significance of hsa_circ_0000520 in CRC and to evaluate its impact on the onset of CRC.

Materials and methods: The levels of hsa_circ_0000520 were measured via quantitative real-time polymerase chain reaction (qRTPCR). To delve into the mechanism through which circ_0000520 impacts CRC and to assess the cellular behavior of CRC cells, a series of experiments including the CCK-8, transwell assay, flow cytometer assay, cell cloning formation, dual-luciferase reporter assay, and bioinformatics method were performed.

Results: The expression levels of hsa_circ_0000520 were markedly elevated in CRC cells and tissue specimens, and this elevation was correlated with a low survival rate. hsa_circ_0000520 affected CRC cell function via the miR-542-3p/MYH9 axis, thus exacerbating cancer progression.

Conclusion: hsa_circ_0000520 functions as a predictive biomarker for the prognosis of CRC and participates in its progression. hsa_ circ_0000520 emerges as a new treatment strategy for CRC patients.