{"title":"Anti-Colon Cancer Effects of Lysate from Potential Probiotic Yeast Strains Derived from Human Breast Milk.","authors":"Yi Wu, Chen Li, Zhiyuan Geng, Lihong Wang, Shufeng Wang, Dingxing Zhang, Dongxu Li, Fanyu Meng","doi":"10.1007/s12602-024-10410-4","DOIUrl":null,"url":null,"abstract":"<p><p>Breast milk contains a diverse array of symbiotic microorganisms, including beneficial probiotics. While most research has focused on the bacterial composition of breast milk, the fungal community (mycobiota) has received limited attention. In this study, we isolated and evaluated yeast strains from breast milk, demonstrating the safety and probiotic potential of four strains through hemolysis tests, antimicrobial activity, tolerance to gastrointestinal conditions, aggregation, cell adhesion, and antioxidant activity assays. These yeast strains significantly inhibited the proliferation of pathogenic bacteria and showed strong antioxidant properties. Moreover, lysates from Rhodotorula mucilaginosa and Cryptococcus laurentii isolated from breast milk markedly inhibited the proliferation of colon cancer cell lines (HT-29 and SW620) and induced apoptosis by upregulating key apoptosis-related genes (caspase 3, caspase 9, and Bax), as well as autophagy-related genes (Beclin-1, LC3, and ATG5). This study provides valuable insights into the potential of breast milk-derived yeast strains for developing novel probiotic therapies for colon cancer treatment.</p>","PeriodicalId":20506,"journal":{"name":"Probiotics and Antimicrobial Proteins","volume":" ","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Probiotics and Antimicrobial Proteins","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12602-024-10410-4","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Breast milk contains a diverse array of symbiotic microorganisms, including beneficial probiotics. While most research has focused on the bacterial composition of breast milk, the fungal community (mycobiota) has received limited attention. In this study, we isolated and evaluated yeast strains from breast milk, demonstrating the safety and probiotic potential of four strains through hemolysis tests, antimicrobial activity, tolerance to gastrointestinal conditions, aggregation, cell adhesion, and antioxidant activity assays. These yeast strains significantly inhibited the proliferation of pathogenic bacteria and showed strong antioxidant properties. Moreover, lysates from Rhodotorula mucilaginosa and Cryptococcus laurentii isolated from breast milk markedly inhibited the proliferation of colon cancer cell lines (HT-29 and SW620) and induced apoptosis by upregulating key apoptosis-related genes (caspase 3, caspase 9, and Bax), as well as autophagy-related genes (Beclin-1, LC3, and ATG5). This study provides valuable insights into the potential of breast milk-derived yeast strains for developing novel probiotic therapies for colon cancer treatment.
期刊介绍:
Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.