Insights into the in vitro biological properties of Australian beach-cast brown seaweed phenolics

IF 3.5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Vigasini Subbiah, Faezeh Ebrahimi, Xinyu Duan, Osman Tuncay Agar, Colin J. Barrow, Hafiz A. R. Suleria
{"title":"Insights into the in vitro biological properties of Australian beach-cast brown seaweed phenolics","authors":"Vigasini Subbiah,&nbsp;Faezeh Ebrahimi,&nbsp;Xinyu Duan,&nbsp;Osman Tuncay Agar,&nbsp;Colin J. Barrow,&nbsp;Hafiz A. R. Suleria","doi":"10.1002/fsn3.4415","DOIUrl":null,"url":null,"abstract":"<p>Five Australian seaweed species, <i>Phyllosphora comosa</i>, <i>Ecklonia radiata</i>, <i>Durvillaea potatorum</i>, <i>Sargassum fallax</i>, and <i>Cystophora siliquosa</i>, thrive along the country's shorelines. Some of these seaweeds have recognized health benefits but have not been fully investigated in terms of their bioactive components and mechanisms of action. We employed ultrasonication with 70% methanol to extract phenolic compounds from these seaweeds and investigated a range of bioactivities for these extracts, including anti-inflammatory activity exploring urease inhibition, nitric oxide scavenging activity, protein denaturation inhibition, and protease inhibition. Anti-diabetic activities were investigated using α-amylase and α-glucosidase inhibition assays. Anti-proliferative and anti-mitotic activities were evaluated using yeast-cell and green-gram models, respectively. Our findings showed that <i>C. siliquosa</i> inhibited nitric oxide, urease, and protease activities, with <i>S. fallax</i>, <i>P. comosa</i>, and <i>E. radiata</i> exhibiting substantial inhibition of protein denaturation. <i>E. radiata</i> displayed inhibitory effects on both α-amylase and α-glucosidase, whereas <i>P. comosa</i> targeted only the α-glucosidase enzyme, indicating different mechanisms of anti-diabetic activity. In these anti-mitotic assays, <i>C. siliquosa</i> exhibited low cell viability and a significant anti-proliferative effect, particularly within 24 h, while <i>E. radiata</i> demonstrated notable inhibition at 48 h. LC-ESI-QTOF-MS/MS investigation identified 48 phenolic compounds, including 19 phenolic acids, 20 flavonoids, and 9 other polyphenols. The presence of these compounds in extracts correlated with observed biological activities. These results support the potential health benefits of these seaweeds and link this activity to the presence of bioactive phenolics.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"12 11","pages":"8956-8967"},"PeriodicalIF":3.5000,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.4415","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Science & Nutrition","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/fsn3.4415","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Five Australian seaweed species, Phyllosphora comosa, Ecklonia radiata, Durvillaea potatorum, Sargassum fallax, and Cystophora siliquosa, thrive along the country's shorelines. Some of these seaweeds have recognized health benefits but have not been fully investigated in terms of their bioactive components and mechanisms of action. We employed ultrasonication with 70% methanol to extract phenolic compounds from these seaweeds and investigated a range of bioactivities for these extracts, including anti-inflammatory activity exploring urease inhibition, nitric oxide scavenging activity, protein denaturation inhibition, and protease inhibition. Anti-diabetic activities were investigated using α-amylase and α-glucosidase inhibition assays. Anti-proliferative and anti-mitotic activities were evaluated using yeast-cell and green-gram models, respectively. Our findings showed that C. siliquosa inhibited nitric oxide, urease, and protease activities, with S. fallax, P. comosa, and E. radiata exhibiting substantial inhibition of protein denaturation. E. radiata displayed inhibitory effects on both α-amylase and α-glucosidase, whereas P. comosa targeted only the α-glucosidase enzyme, indicating different mechanisms of anti-diabetic activity. In these anti-mitotic assays, C. siliquosa exhibited low cell viability and a significant anti-proliferative effect, particularly within 24 h, while E. radiata demonstrated notable inhibition at 48 h. LC-ESI-QTOF-MS/MS investigation identified 48 phenolic compounds, including 19 phenolic acids, 20 flavonoids, and 9 other polyphenols. The presence of these compounds in extracts correlated with observed biological activities. These results support the potential health benefits of these seaweeds and link this activity to the presence of bioactive phenolics.

Abstract Image

深入了解澳大利亚海滩投投褐藻酚类物质的体外生物学特性
五种澳大利亚海藻,叶藻,Ecklonia radiata, Durvillaea potatorum,马尾藻和Cystophora silquosa,在这个国家的海岸线上茁壮成长。其中一些海藻已被公认对健康有益,但其生物活性成分和作用机制尚未得到充分研究。我们采用70%甲醇超声提取这些海藻中的酚类化合物,并研究了这些提取物的一系列生物活性,包括抗炎活性,探索脲酶抑制活性,一氧化氮清除活性,蛋白质变性抑制活性和蛋白酶抑制活性。采用α-淀粉酶和α-葡萄糖苷酶抑制试验研究其抗糖尿病活性。分别用酵母细胞模型和格林克模型评价其抗增殖和抗有丝分裂活性。我们的研究结果表明,水果糖可以抑制一氧化氮、脲酶和蛋白酶的活性,而黄花蓟马、comosa和辐射蓟马对蛋白质变性有明显的抑制作用。辐射藤对α-淀粉酶和α-葡萄糖苷酶均有抑制作用,而青花藤仅对α-葡萄糖苷酶有抑制作用,提示其抗糖尿病作用机制不同。在这些抗有丝分裂实验中,硅藻土表现出较低的细胞活力和显著的抗增殖作用,特别是在24 h内,而辐射木在48 h时表现出显著的抑制作用。LC-ESI-QTOF-MS/MS鉴定了48种酚类化合物,包括19种酚酸、20种黄酮类化合物和9种其他多酚类化合物。这些化合物在提取物中的存在与观察到的生物活性相关。这些结果支持这些海藻的潜在健康益处,并将这种活性与生物活性酚类物质的存在联系起来。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Food Science & Nutrition
Food Science & Nutrition Agricultural and Biological Sciences-Food Science
CiteScore
7.40
自引率
5.10%
发文量
434
审稿时长
24 weeks
期刊介绍: Food Science & Nutrition is the peer-reviewed journal for rapid dissemination of research in all areas of food science and nutrition. The Journal will consider submissions of quality papers describing the results of fundamental and applied research related to all aspects of human food and nutrition, as well as interdisciplinary research that spans these two fields.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信