Investigation for Regulation of a DNA-Programmed Bimetallic Nanozyme and Its Biosensing Applications

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2024-11-30 DOI:10.1021/acs.analchem.4c05241

Jiaxuan Xiao, Xiaofeng Yang, Xinshuo Zhang, Xiangheng Niu, Yujia Guo, Nuanfei Zhu, Kun Zeng, Zhen Zhang

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引用次数: 0

Abstract

The DNA-mediated growth strategy of bimetallic nanozymes is considered as an effective approach to regulate their peroxidase activity via tuning the morphology and nanostructure. Albeit important, its biosensing application in rational methods’ design and performance improvement is limited due to the deficiency of a systematic understanding of the interactions between DNA and nanomaterials used. Herein, four homo-oligonucleotides as capping ligands were employed to functionalize the bimetallic nanozymes, where Pt nanoparticles (PtNPs) were in situ synthesized onto DNA-bound Au nanorods (AuNRs), and the effects of DNA with different lengths on the state of bimetallic nanozymes were investigated in detail. It was found that the aggregation of AuNRs obviously depended on the variety and number of DNA oligonucleotides with the absorbance ratio at 810 and 525 nm (A810/A525), ranking as follows: AuNRs/A10/PtNPs > AuNRs/G10/PtNPs > AuNRs/C10/PtNPs ≫ AuNRs/T10/PtNPs, which is consistent with the value of K_m for TMB, indicating that the dispersal/aggregation of the AuNRs is closely related to the deposition and growth of PtNPs, thereby significantly influencing their peroxidase activity. According to our discoveries, a novel colorimetric array platform was fabricated using the above four types of DNA-encoded Pt–Au bimetallic nanozymes as sensing elements for sensitively discriminating the five biological thiols (l-cys, GSH, Hcy, DTT, and Cys–Gly) and identifying the normal cells/tumor cells, respectively. Our work provides a new insight into DNA-programmed bimetallic nanozyme regulation and broadens its sensing applications.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.