Highly Efficient and Long-Lasting Chemiluminescence-Functionalized Nanohydrogel for Imaging-Guided Precise Piperlongumine Chemotherapy.

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Miaomiao Zhang, Kang Wang, Meiqin Li, Xun Fang, Zhongxiang Chen, Yuting Li, Haifeng Lu, Qunlin Zhang
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引用次数: 0

Abstract

A major challenge for imaging-guided precise chemotherapy remains the ability to track the in situ real-time variation of the reactive oxygen species (ROS) level during treatment with prooxidation antitumor drugs. Chemiluminescence (CL) is widely used as an in vivo imaging tool with an excellent signal-to-noise ratio and high biological safety. However, suffering from flash-type and poor water solubility, most of the reported CL probes for ROS detection are unsuitable for in vivo long-term tracking. Herein, we designed a water-soluble CL nanohydrogel (L-012/Co2+@NGs) by cross-linking of vinyl-derived β-cyclodextrin monomer (MAH-β-CD) and loaded with luminol analog L-012 and cobalt ions (Co2+). In vitro studies reveal that L-012/Co2+@NGs exhibit long-lasting CL emission (up to 4 h) due to the slow diffusion of hydrogen peroxide in the nanohydrogel. High catalytic efficiency from the accelerated reduction of Co3+ to Co2+ through Tris and chelation of Co2+, as well as protection of the β-CD cavity against the active intermediate of L-012, enables L-012/Co2+@NGs to exhibit a 722-fold CL signal turn-on ratio and a nanomolar limit of detection (8.9 nmol/L). Piperlongumine (PL) was selected as a model of prooxidation drugs. The long-term and highly efficient CL strategy was designed for monitoring the local dynamic changes of ROS in PL-treated tumor-bearing mice for 150 min. The CL signal increased over time until reaching its maximum with a ∼6-fold increase at 15 min and then decreased slowly. The CL-functionalized nanohydrogel platform with good biocompatibility offers a great opportunity for imaging-guided precise tumor chemotherapy of PL and other prooxidation antitumor drugs.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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