Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas.

IF 2.7 3区医学 Q3 NEUROSCIENCES

Brain Sciences Pub Date : 2024-11-20 DOI:10.3390/brainsci14111157

László Szivos, József Virga, Zoltán Mészár, Melinda Rostás, Andrea Bakó, Gábor Zahuczki, Tibor Hortobágyi, Álmos Klekner

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引用次数: 0

Abstract

Background: Astrocytoma, an IDH-mutant is a common primary brain tumor. Total surgical resection is not feasible due to peritumoral infiltration mediated by extracellular matrix (ECM) molecules.

Methods: This study aimed at determining the expression pattern of ECM molecules in different prognostic groups of WHO grade 2 and grade 3 patients and identifying the effect of onco-radiotherapy on tumor cell invasion of grade 3 patients. Gene and protein expression of ECM molecules was determined by qRT-PCR and immunohistochemistry, respectively.

Results: In the different prognostic groups of grade 2 tumors HMMR, IDH-1, MKI-67, PDGF-A and versican, in grade 3 tumors integrin α-3, and in both groups integrin α-3 and IDH-1 mRNA expression was significantly different. Regarding protein expression, only integrin αV expression changed significantly in the prognostic groups of grade 2 tumors.

Conclusions: Based on the invasion spectrum determined by this joint gene and protein expression analysis, there was a sensitivity of 87.5% and a negative predictive value of 88.9% regarding the different prognostic groups of grade 2 astrocytoma. For grade 3 tumors, the applied standard oncotherapeutic modalities apparently lacked significant anti-invasive effects.

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扩散浸润的 2 级和 3 级星形细胞瘤中与侵袭相关的细胞外基质分子的预后作用

背景：IDH 突变的星形细胞瘤是一种常见的原发性脑肿瘤：IDH突变的星形细胞瘤是一种常见的原发性脑肿瘤。由于细胞外基质（ECM）分子介导的瘤周浸润，完全手术切除并不可行：本研究旨在确定 ECM 分子在不同预后组 WHO 2 级和 3 级患者中的表达模式，并确定联合放疗对 3 级患者肿瘤细胞侵袭的影响。ECM分子的基因和蛋白表达分别通过qRT-PCR和免疫组化进行检测：结果：在不同的预后组中，2级肿瘤的 HMMR、IDH-1、MKI-67、PDGF-A 和 versican，3级肿瘤的整合素 α-3，以及两组整合素 α-3 和 IDH-1 mRNA 表达均有显著差异。在蛋白质表达方面，只有整合素αV的表达在2级肿瘤的预后组中有明显变化：根据基因和蛋白表达联合分析确定的侵袭谱，2级星形细胞瘤不同预后组别的敏感性为87.5%，阴性预测值为88.9%。对于3级肿瘤，所采用的标准肿瘤治疗方法显然缺乏显著的抗侵袭效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain Sciences Neuroscience-General Neuroscience

CiteScore

4.80

自引率

9.10%

发文量

1472

审稿时长

18.71 days

期刊介绍： Brain Sciences (ISSN 2076-3425) is a peer-reviewed scientific journal that publishes original articles, critical reviews, research notes and short communications in the areas of cognitive neuroscience, developmental neuroscience, molecular and cellular neuroscience, neural engineering, neuroimaging, neurolinguistics, neuropathy, systems neuroscience, and theoretical and computational neuroscience. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.