Degradation Kinetics and Mechanism of β-Cypermethrin and 3-Phenoxybenzoic Acid by Lysinibacillus pakistanensis VF-2 in Soil Remediation

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2024-11-27 DOI:10.1021/acs.jafc.4c08344

Xuan Yu, Hu Zhou, Jie Tang, Chuanning Peng, Siqi Chen, Siqi Huan, Qi Wen, Yingyue Zhang, Wenliang Xiang, Xuejiao Chen, Qing Zhang

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Abstract

Pyrethroid pesticide residues have detrimental effects on soil ecology and crop growth during insecticidal operations in agriculture. In this study, a novel strain Lysinibacillus pakistanensis VF-2 was isolated from long-term pesticide-treated cropland and had a maximum degradation efficiency of 81.66% for synthetic pyrethroid β-cypermethrin (β-CY) under optimized conditions. The analysis of intermediate products revealed that the degradation pathway of β-CY mainly involves ester bond hydrolysis, diphenyl ether decomposition, and phthalate ester degradation. Whole-genome sequencing and RT-qPCR analysis revealed the involvement of carboxylesterases, dioxygenases, and aromatic compound degrading enzymes in the degradation of β-CY. In the soil bioaugmentation experiment, the strain VF-2 can synergistically interact with indigenous microorganisms, significantly enhancing the degradation efficiency of β-CY and its metabolite 3-phenoxybenzoic acid (3-PBA) from 17.08% and 7.62% to 73.46% and 62.29%, respectively. This study suggests that strain VF-2 is a promising candidate for in situ coremediation of pyrethroid and intermediate metabolite residues in soil.

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巴基斯坦溶血杆菌 VF-2 在土壤修复中降解 β-氯氰菊酯和 3-苯氧基苯甲酸的动力学和机制

在农业杀虫过程中，拟除虫菊酯农药残留会对土壤生态和作物生长产生不利影响。本研究从长期施用农药的耕地中分离出一株新型的巴基斯坦溶血杆菌（Lysinibacillus pakistanensis VF-2），在优化条件下，该菌株对合成拟除虫菊酯 β-氯氰菊酯（β-CY）的降解效率最高达 81.66%。对中间产物的分析表明，β-CY 的降解途径主要包括酯键水解、二苯醚分解和邻苯二甲酸酯降解。全基因组测序和 RT-qPCR 分析表明，羧酸酯酶、二氧酶和芳香族化合物降解酶参与了 β-CY 的降解过程。在土壤生物增殖实验中，菌株 VF-2 能与本地微生物产生协同作用，显著提高 β-CY 及其代谢物 3-苯氧基苯甲酸（3-PBA）的降解效率，分别从 17.08% 和 7.62% 提高到 73.46% 和 62.29%。这项研究表明，菌株 VF-2 是对土壤中残留的拟除虫菊酯和中间代谢物进行原位核心调解的理想候选菌株。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.