Copper-based nanozyme-linked immunosorbent assay for quantitative detection of fumonisin B1

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2024-11-23 DOI:10.1016/j.jfca.2024.107012

Fen Tang , Qi Guo , Lina Fu , Yajuan Huang , Zhaowei Zhang , Peiwu Li , Li Yu

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引用次数: 0

Abstract

Fumonisin B₁ (FB₁) is a Group 2B carcinogen. It is vital to develop an ultra-sensitive, low-cost, and rapid screening method for FB₁ for food safety risk control. In this study, a novel copper-based nanozyme was synthesized. The anti-FB₁ monoclonal antibody (mAb) was conjugated with copper-based nanozyme through physical adsorption to probe construction. The probe could compete with the limited antigen and trigger a color change when employing the 3,3’,5,5’-tetramethylbenzidine (TMB) as chromogenic substrate in the presence of H₂O₂. Several experimental conditions were optimized, including the concentration of coating antigen, the concentration of mAb coupled to the nanozyme, incubation time, and methanol concentration. Under optimal conditions, a direct competitive nanozyme-linked immunosorbent assay (NLISA) has been established with a limit of detection (LOD) of 6.52 pg/mL. The developed method presented good accuracy and precision. The spiked recoveries ranged from 97.368 % to 120.916 %, with coefficient of variation (CV) below 10 %. In addition, there was a high consistency (R²=0.9820 for corn; R²=0.9936 for wheat) between the developed detection method and the reference method, liquid chromatography/mass spectrometry (LC-MS/MS). This research can expand the application areas of copper-based nanozymes and improve the sensitivity and efficiency of FB₁ monitoring.

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定量检测伏马菌毒素 B1 的铜基纳米酶联免疫吸附分析法

伏马菌毒素 B1 (FB1) 是一种 2B 类致癌物质。为控制食品安全风险，开发一种超灵敏、低成本的 FB1 快速筛查方法至关重要。本研究合成了一种新型铜基纳米酶。抗 FB1 单克隆抗体（mAb）通过物理吸附与铜基纳米酶共轭，构建探针。该探针能与有限的抗原竞争，并在 H2O2 存在下以 3,3',5,5'-四甲基联苯胺（TMB）为显色底物引发颜色变化。我们优化了几种实验条件，包括包被抗原的浓度、与纳米酶偶联的 mAb 浓度、孵育时间和甲醇浓度。在最佳条件下，建立了一种直接竞争性纳米酶联免疫吸附测定法（NLISA），其检测限（LOD）为 6.52 pg/mL。所开发的方法具有良好的准确度和精密度。加标回收率为 97.368 % 至 120.916 %，变异系数（CV）低于 10 %。此外，所开发的检测方法与参考方法液相色谱/质谱（LC-MS/MS）之间具有很高的一致性（玉米的 R2=0.9820；小麦的 R2=0.9936）。该研究可拓展铜基纳米酶的应用领域，提高 FB1 监测的灵敏度和效率。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.