Chemically induced proximity reveals a Piezo-dependent meiotic checkpoint at the oocyte nuclear envelope

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2024-11-22 DOI:10.1126/science.adm7969

Chenshu Liu, Abby F. Dernburg

引用次数: 0

Abstract

Sexual reproduction relies on robust quality control during meiosis. Assembly of the synaptonemal complex between homologous chromosomes (synapsis) regulates meiotic recombination and is crucial for accurate chromosome segregation in most eukaryotes. Synapsis defects can trigger cell cycle delays and, in some cases, apoptosis. We developed and deployed a chemically induced proximity system to identify key elements of this quality control pathway in Caenorhabditis elegans. Persistence of the polo-like kinase PLK-2 at pairing centers—specialized chromosome regions that interact with the nuclear envelope—induced apoptosis of oocytes in response to phosphorylation and destabilization of the nuclear lamina. Unexpectedly, the Piezo1/PEZO-1 channel localized to the nuclear envelope and was required to transduce this signal to promote apoptosis in maturing oocytes.

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化学诱导的接近性揭示了卵母细胞核膜上依赖压电效应的减数分裂检查点。

有性生殖依赖于减数分裂过程中强有力的质量控制。同源染色体之间突触复合体的组装（突触）调节减数分裂重组，对大多数真核生物的染色体准确分离至关重要。突触缺陷可引发细胞周期延迟，在某些情况下还会导致细胞凋亡。我们开发并部署了一个化学诱导接近系统，以鉴定秀丽隐杆线虫中这一质量控制途径的关键要素。类磷酸化激酶 PLK-2 在配对中心--与核膜相互作用的特殊染色体区域--的持续存在诱导了卵母细胞的凋亡，以响应核膜的磷酸化和不稳定性。意想不到的是，Piezo1/PEZO-1通道定位在核包膜上，并需要传递这一信号以促进成熟卵母细胞的凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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