Improvement and Recovery of Intestinal Flora Disorder Caused by Ciprofloxacin Using Lactic Acid Bacteria.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Probiotics and Antimicrobial Proteins Pub Date : 2024-11-20 DOI:10.1007/s12602-024-10401-5

Xiumin Su, Li Su, Mengyuan Cao, Yulu Sun, Jinghan Dai, Yuanjie He, Wei Li, Wupeng Ge, Xin Lv, Qiang Zhang, Shenghui Cui, Jia Chen, Baowei Yang

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Abstract

In this study, four lactic acid bacteria (LAB) strains demonstrating ciprofloxacin, bile salt, gastric fluid, and intestinal fluid tolerance as well as adhesion ability to Caco-2 and HT-29 cells were used to improve and recover the intestinal flora disorders caused by ciprofloxacin, among which, Lactobacillus brevis 505 exhibited excellent adhesion ability to two kinds of cells and colonization ability to mouse intestinal. After ciprofloxacin treatment, certain recovery effect on cecum caused by ciprofloxacin in the mice was found during natural recovery (group 5C2), but it was challenging to fully restore the intestinal integrity to the initial level. After L. brevis 505 intervention (group 5C5), the intestinal damage to the colon and ileum caused by ciprofloxacin in mice was significantly alleviated; the recovery effect was better than that of natural recovery. Additionally, L. brevis 505 could effectively regulate INF-γ, sIgA, and RegIIIγ increase induced by ciprofloxacin. Shannon and Simpson index of the intestinal flora of mice in 5C5 group were higher than those in other group, the relative abundance of Bifidobacterium and Lactobacillus in the mice in 5C5 group was increased, indicating that LAB can better restore the structure and abundance of intestinal microflora. Consequently, L. brevis 505 shows promise as a probiotic for gut microbiota restoration and rebuilding during antibiotic therapy.

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利用乳酸菌改善和恢复环丙沙星引起的肠道菌群失调。

本研究利用对环丙沙星、胆盐、胃液和肠液耐受性以及对Caco-2和HT-29细胞粘附能力均较强的4株乳酸菌（LAB）来改善和恢复环丙沙星引起的肠道菌群失调，其中布氏乳杆菌505对两种细胞的粘附能力和对小鼠肠道的定植能力均较强。环丙沙星治疗后，在自然恢复期（5C2 组）发现环丙沙星对小鼠盲肠有一定的恢复作用，但要将肠道完整性完全恢复到初始水平仍有难度。在对 L. brevis 505 进行干预后（5C5 组），环丙沙星对小鼠结肠和回肠造成的肠道损伤明显减轻，恢复效果优于自然恢复。此外，L. brevis 505 还能有效调节环丙沙星诱导的 INF-γ、sIgA 和 RegIIIγ 的升高。5C5组小鼠肠道菌群的香农指数和辛普森指数高于其他组，5C5组小鼠体内双歧杆菌和乳酸杆菌的相对丰度增加，这表明 LAB 能更好地恢复肠道微生物菌群的结构和丰度。因此，L. brevis 505有望成为抗生素治疗期间恢复和重建肠道微生物群的益生菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.