Enhanced creaminess of plant-based milk via enrichment of papain hydrolyzed oleosomes

Abstract

There is an increased consumer demand for plant-based milk in substituting dairy milk due to the ethical, health concerns and environmentally-friendly choice. However, perceived creaminess as dominant attributes present a big challenge in consumer acceptance for those milk alternatives. In this study, we developed a novel and easily scalable strategy to enhance the creaminess of soy milk via enrichment of oleosomes. The soybean oleosome creams were extracted and hydrolyzed with papain, resulting in formation of oil droplets with more phospholipid and less protein at the surface, which significantly reduce friction coefficient in the presence of saliva (from 0.15 to 0.03 at a speed around 50 mm/s). Moreover, blending papain-hydrolyzed oleosome creams with raw soy milk enables the creation of a plant-based milk that matches the nutritional profile, lubrication properties, and creaminess of full-fat dairy milk. QCM-D and passive microrheology were employed to characterize hydration of oleosomes into the mucin layer and relevant viscosity change, suggesting that papain hydrolyzed oleosome might decrease friction coefficient via hydration lubrication mechanism. This approach could be applied to enhance the creaminess mouthfeel and nutritional profile of plant-based milk.