Combined transcriptome and metabolome analysis reveals the mechanism of fruit quality formation in different watermelon (Citrullus lanatus) cultivars

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2024-11-14 DOI:10.1016/j.scienta.2024.113797

Zhenzhen Peng , Shuhui Song , Daqi Fu , Jiahua Zhou , Hong Chang , Baogang Wang , Ranran Xu , Chao Zhang , Yunxiang Wang

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引用次数: 0

Abstract

Fruit characteristics (flesh color, sweetness, flavor, etc.) of watermelon are largely the result of human selection. In this study, we combined the metabolome and transcriptome to investigate differentially expressed genes and differential metabolites related to sugar-acid and flesh color in four commercial watermelon cultivars. Fifty-eight genes and nine metabolites were identified in the organic acid and sugar metabolism pathways. Six of these metabolites were significantly up-regulated in ‘Jingcai No.1’ (JC), including sucrose, fructose, cellobiose, oxaloacetate, succinate, and malate. The expression of sucrose synthase 2 (SUS2), sucrose-phosphate synthase (SPS1), fructose bisphosphate aldolase (FBA2), glyceraldehyde 3-phosphate dehydrogenase(GAPN), trehalose-phosphate phosphatase (TPPJ), trehalose-phosphate synthase (TPS1), 1,4-alpha-glucan-branching (SBE1) and SBE3, which are involved in sugar and acid metabolism, was also significantly higher than that of the other three cultivars. A total of 55 genes and 102 metabolites were identified in the pathway related to flesh color formation (carotenoid synthesis, phenylpropanoid synthesis, flavonoid synthesis). The abundance of genes and metabolites associated with sugar and acid metabolism and color formation were generally lower in ‘Jingmei 2k’ (EK) and ‘L600’ (LB) than in JC and ‘Chaoyue’ (CY). In summary, the omics analysis of the four watermelon cultivars revealed significant differences in metabolic genes and metabolites involved in sugar-acid biosynthesis and flesh color formation, which provides new ideas for improving the quality and commercial value of watermelon fruits through genetic engineering.

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转录组和代谢组联合分析揭示了不同西瓜（Citrullus lanatus）栽培品种果实品质形成的机理

西瓜的果实特征（瓤色、甜度、风味等）在很大程度上是人类选择的结果。在本研究中，我们结合代谢组和转录组研究了四个商业西瓜栽培品种中与糖酸和果肉颜色相关的差异表达基因和差异代谢产物。在有机酸和糖代谢途径中发现了 58 个基因和 9 种代谢物。其中 6 种代谢物在 "京彩 1 号"（JC）中明显上调，包括蔗糖、果糖、纤维生物糖、草酰乙酸、琥珀酸和苹果酸。蔗糖合成酶 2（SUS2）、蔗糖磷酸合成酶（SPS1）、果糖二磷酸醛缩酶（FBA2）、3-磷酸甘油醛脱氢酶（GAPN）、三卤糖磷酸酶（TPPJ）、果糖二磷酸醛缩酶（FBA2）、3-磷酸甘油醛脱氢酶（GAPN此外，参与糖和酸代谢的三聚磷酸酯合成酶（TPS1）、1,4-α-葡聚糖支链（SBE1）和 SBE3 的含量也明显高于其他三个品种。在与果肉颜色形成有关的途径（类胡萝卜素合成、苯丙酮合成、黄酮合成）中，共鉴定出 55 个基因和 102 个代谢物。与糖、酸代谢和颜色形成相关的基因和代谢物的丰度在'京梅 2k'（EK）和'L600'（LB）中普遍低于 JC 和'Chaoyue'（CY）。综上所述，对四个西瓜栽培品种的omics分析表明，参与糖酸生物合成和果肉颜色形成的代谢基因和代谢产物存在显著差异，这为通过基因工程提高西瓜果实的品质和商业价值提供了新思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.