Effects of Diets With Different Carbohydrate to Lipid Ratios on the Growth Performance, Ion Transport, and Carbohydrate, Lipid and Ammonia Metabolism of Nile Tilapia (Oreochromis niloticus) Under Long-Term Saline–Alkali Stress

Abstract

A 50-day test was adopted to compare the growth performance, liver histology, glucose metabolism, lipid (L) metabolism, ion transport, and ammonia metabolism of tilapia fed different carbohydrate–lipid (C:L) ratio diets under saline–alkaline water (salinity = 16 mmol/L and alkalinity = 35 mmol/L). The C and L levels of five isoenergetic (16.5 kJ/g) and isonitrogenous (32% protein) diets were C45%:L3% (L3), C38%:L6% (L6), C31%:L9% (L9), C24%:L12% (L12), and C17%:L15% (L15). This study found that the dietary C:L ratio did not affect the survival rate (SR), feed conversion ratio (FCR), or condition factor of tilapia in saline–alkali water, but fish in the L12 group had the highest weight gain (WG) rate and the lowest hepatosomatic index (HSI) compared with the other groups. Fish fed the higher C diet (L3 and L6) had a higher ion transport capacity and ammonia excretion capacity in gills. However, the highest mRNA expression of genes involved in glutamine metabolism and urea metabolism in the liver was found in the high-L diet groups (L12 and L15). In particular, a lower serum ammonia concentration was observed in the high-L diet groups (L12 and L15). In addition, biochemical indicators indicated that the L12 group had the highest liver pyruvic acid, lactic dehydrogenase (LDH), and lipase (LPS) and serum total cholesterol (T-CHO) contents. In summary, this study indicated that dietary Ls could promote glutamine metabolism and urea metabolism more than dietary Cs and then reduce the serum ammonia concentration of tilapia in saline–alkali water. A dietary C:L ratio of 2:1 was beneficial to the growth and ammonia excretion of tilapia in saline–alkali water in this study.