Acetylcholinesterase-free colorimetric sensing platform for carbosulfan detection based on hollow PDA/MnO2 nanozyme

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-11-15 DOI:10.1016/j.foodchem.2024.142075

Jingfang Guo, Feng Zhao, Zhenhui Yue, Zhen Lei

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Abstract

Rapid and accurate detection of carbosulfan residues in vegetables is important for ensuring food safety. Herein, based on the unique hydrolysis behavior of carbosulfan, a nanozyme-based colorimetric sensing platform was proposed for detection of carbosulfan. Hollow polydopamine/MnO₂ nanoparticles (H-PDA/MnO₂ NPs) with excellent oxidase-like activity were synthesized, which can promote the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidative product (oxTMB). Under acidic conditions, carbosulfan can be decomposed and produce reductive sulfide species (-SH), which are capable to disintegrate MnO₂ NPs, resulting in decreased oxidase-like activity of H-PDA/MnO₂ NPs. Based on the inhibitory effect on oxidase activity of H-PDA/MnO₂ NPs, an acetylcholinesterase-free colorimetric assay was proposed for detection of carbosulfan with low limit of detection of 0.63 ng mL⁻¹. Integrating test swabs with smartphone, a portable colorimetric sensor was constructed, showing great potential for on-site detection. To demonstrate the feasibility of this method, carbosulfan in real vegetable samples were determined.

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基于中空 PDA/MnO2 纳米酶的无乙酰胆碱酯酶硫丹检测比色传感平台

快速准确地检测蔬菜中的硫丹残留对确保食品安全非常重要。本文基于硫丹独特的水解行为，提出了一种基于纳米酶的比色传感平台来检测硫丹。研究人员合成了具有优异氧化酶样活性的中空多巴胺/MnO2纳米颗粒（H-PDA/MnO2 NPs），它能促进3,3′,5,5′-四甲基联苯胺（TMB）氧化成蓝色氧化产物（oxTMB）。在酸性条件下，硫丹可被分解并产生还原性硫化物（-SH），这些硫化物能够分解二氧化锰 NPs，从而导致 H-PDA/MnO2 NPs 的氧化酶样活性降低。基于 H-PDA/MnO2 NPs 对氧化酶活性的抑制作用，提出了一种不含乙酰胆碱酯酶的比色法检测硫丹，其检测限低至 0.63 ng mL-1。将测试拭子与智能手机相结合，构建了一种便携式比色传感器，显示出现场检测的巨大潜力。为了证明该方法的可行性，测定了真实蔬菜样品中的硫丹含量。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.