The effect of miR-205a with RUNX2 towards proliferation and differentiation of chicken chondrocytes in thiram-induced tibial dyschondroplasia

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-11-08 DOI:10.1016/j.psj.2024.104535

Yuxin Zhou , Yuxiang Lu , Hengyong Xu , Xuyang Ji , Qingqing Deng , Xi Wang , Yao Zhang , Qiuhang Li , Yusheng Lu , Alma Rustempasic , Yiping Liu , Yan Wang

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引用次数: 0

Abstract

Tibial dyschondroplasia (TD) is a kind of metabolic bone disease in fast-growing broilers, which seriously restricts the development of poultry industry. Our previous studies have revealed a significant upregulation of miR-205a in TD cartilage tissue, suggesting its potential role as a regulatory factor in the pathogenesis of TD. However, the precise function implications and underlying regulatory mechanism remain elusive. Therefore, this study aims to elucidate the biological functions and regulatory mechanisms of miR-205a in the progression of TD by employing mehtodologies such as qRT-PCR, CCK-8 assay, EdU assays, and flow cytometry. The findings demonstrated that the transfection of miR-205a overexpression plasmid reduced chondrocytes growth and development in TD while enhancing apoptosis; conversely, blocking miR-205a had opposite effects. RUNX2 was identified as a target gene of miR-205a through biosynthesis and dual luciferase assays, and its overexpression helps chondrocytes in TD grow and develop. However, when both miR-205a and RUNX2 were overexpressed, the regulatory effect of RUNX2 was significantly suppressed. In conclusion, miR-205a plays a role in slowing the growth and development of chondrocytes in TD by targeting and reducing RUNX2 expression, which helps to initiate and progress TD.

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miR-205a 与 RUNX2 对噻喃诱导的胫骨软骨发育不良中鸡软骨细胞增殖和分化的影响

胫骨发育不良（TD）是快速生长肉鸡的一种代谢性骨病，严重制约着家禽业的发展。我们之前的研究发现，miR-205a 在 TD 软骨组织中显著上调，这表明它可能是 TD 发病机制中的一个调控因子。然而，其确切的功能影响和潜在的调控机制仍未确定。因此，本研究采用 qRT-PCR、CCK-8 检测、EdU 检测和流式细胞术等方法，旨在阐明 miR-205a 在 TD 进展过程中的生物学功能和调控机制。研究结果表明，转染miR-205a过表达质粒会降低TD软骨细胞的生长发育，同时增强软骨细胞的凋亡；反之，阻断miR-205a则会产生相反的效果。通过生物合成和双荧光素酶检测，RUNX2 被确定为 miR-205a 的靶基因，它的过表达有助于 TD 软骨细胞的生长和发育。然而，当 miR-205a 和 RUNX2 同时过表达时，RUNX2 的调控作用被显著抑制。总之，miR-205a通过靶向和减少RUNX2的表达，在TD软骨细胞的生长发育过程中起着减缓作用，有助于TD的启动和进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.