Protective effects of Juglans regia oil on lead acetate-induced reprotoxicity in rats: an antioxidant, histological and computational molecular study.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2024-11-14 DOI:10.1002/jsfa.14024

Riadh Badraoui, Manel Gargouri, Faten Brahmi, Hmed Ben-Nasr, Insaf Bahrini, Ahlem Soussi

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引用次数: 0

Abstract

Background: Lead (Pb) is a metal that affects many body systems, primarily the male reproductive system. This study aimed to examine the phytochemical profiling and beneficial effects of Juglans regia oil (JRO) in alleviating lead-induced reprotoxicity-associated oxidative injury in rats. Adult male Wistar rats were randomly divided into four groups as follows: control group received no treatment, Pb group was exposed to 0.344 g kg^-1 bw of Pb acetate, Pb + JRO group was co-treated with Pb plus walnut oil (0.9 g kg^-1 bw) and JRO group received walnut oil only.

Results: Pb-treated rats showed significantly decreased gonado-somatic index, count and viability of sperm. Testosterone levels decreased in Pb-treated animals. Besides, Pb disrupted the oxidative/antioxidative status, the plasmatic lipase activity and testicular Pb content. Furthermore, Pb produced testis histopathological features, especially cell degeneration, atrophy and tubular disarrangement. Co-treatment with JRO was found to be effective for recovering the increased testicular oxidative damage-associated histological features in the Pb group. This was supported by the in silico modeling results.

Conclusion: The in silico analyses revealed that JRO compounds bound androgen receptor and RAC-alpha serine/threonine kinase with high affinities, which together with pharmacokinetic properties and molecular interactions satisfactorily support the beneficial in vivo findings in the Pb + JRO group of rats. © 2024 Society of Chemical Industry.

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胡桃油对醋酸铅诱导的大鼠生殖毒性的保护作用：一项抗氧化、组织学和计算分子研究。

背景：铅（Pb）是一种会影响许多身体系统（主要是男性生殖系统）的金属。本研究旨在检测植物化学成分分析以及胡桃油（JRO）在减轻铅诱导的大鼠生殖毒性相关氧化损伤方面的有益作用。成年雄性 Wistar 大鼠被随机分为以下四组：对照组不接受任何处理；铅组暴露于 0.344 g kg-1 bw 的醋酸铅；铅 + JRO 组接受铅加核桃油（0.9 g kg-1 bw）的联合处理；JRO 组仅接受核桃油处理：结果：铅处理大鼠的性腺指数、精子数量和活力明显下降。铅处理动物的睾酮水平也有所下降。此外，铅还破坏了氧化/抗氧化状态、质体脂肪酶活性和睾丸中的铅含量。此外，铅还会产生睾丸组织病理学特征，尤其是细胞变性、萎缩和小管错乱。在铅组中，联合使用 JRO 能有效恢复睾丸氧化损伤相关组织学特征的增加。这一点得到了硅学建模结果的支持：结论：硅学分析表明，JRO化合物与雄激素受体和RAC-α丝氨酸/苏氨酸激酶的结合亲和力很高，再加上药代动力学特性和分子相互作用，可以令人满意地支持铅＋JRO组大鼠体内的有益发现。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.