Adaptive wheel exercise for mouse models of Parkinson’s Disease

IF 2.7 4区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Henry Skelton , Dayton Grogan , Amrutha Kotlure , Ken Berglund , Claire-Anne Gutekunst , Robert Gross
{"title":"Adaptive wheel exercise for mouse models of Parkinson’s Disease","authors":"Henry Skelton ,&nbsp;Dayton Grogan ,&nbsp;Amrutha Kotlure ,&nbsp;Ken Berglund ,&nbsp;Claire-Anne Gutekunst ,&nbsp;Robert Gross","doi":"10.1016/j.jneumeth.2024.110314","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Physical exercise has been extensively studied for its therapeutic properties in neurological disease, particularly Parkinson’s Disease (PD). However, the established techniques for exercise in mice are not well suited to motor-deficient disease-model animals, rely on spontaneous activity or force exercise with aversive stimuli, and do not facilitate active measurement of neurophysiology with tethered assays. Motorized wheel exercise may overcome these limitations, but has not been shown to reliably induce running in mice.</div></div><div><h3>New method</h3><div>We developed an apparatus and technique for inducing exercise in mice without aversive stimuli, using a motorized wheel that dynamically responds to subject performance.</div></div><div><h3>Results</h3><div>A commercially available motorized wheel system did not satisfactorily provide for exercise, as mice tended to avoid running at higher speeds. Our adaptive wheel exercise platform allowed for effective exercise induction in the 6-hydroxydopamine mouse model of PD, including with precise behavioral measurements and synchronized single-unit electrophysiology.</div></div><div><h3>Comparison with existing methods</h3><div>Our approach provides a superior physical platform and programming strategy compared to previously described techniques for motorized wheel exercise. Unlike voluntary exercise, this allows for controlled experimental induction of running, without the use of aversive stimuli that is typical of treadmill-based techniques.</div></div><div><h3>Conclusions</h3><div>Adaptive wheel exercise should allow for physical exercise to be better studied as a dynamic, physiological intervention in parkinsonian mice, as well as other neurological disease models.</div></div>","PeriodicalId":16415,"journal":{"name":"Journal of Neuroscience Methods","volume":"414 ","pages":"Article 110314"},"PeriodicalIF":2.7000,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Neuroscience Methods","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165027024002590","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Background

Physical exercise has been extensively studied for its therapeutic properties in neurological disease, particularly Parkinson’s Disease (PD). However, the established techniques for exercise in mice are not well suited to motor-deficient disease-model animals, rely on spontaneous activity or force exercise with aversive stimuli, and do not facilitate active measurement of neurophysiology with tethered assays. Motorized wheel exercise may overcome these limitations, but has not been shown to reliably induce running in mice.

New method

We developed an apparatus and technique for inducing exercise in mice without aversive stimuli, using a motorized wheel that dynamically responds to subject performance.

Results

A commercially available motorized wheel system did not satisfactorily provide for exercise, as mice tended to avoid running at higher speeds. Our adaptive wheel exercise platform allowed for effective exercise induction in the 6-hydroxydopamine mouse model of PD, including with precise behavioral measurements and synchronized single-unit electrophysiology.

Comparison with existing methods

Our approach provides a superior physical platform and programming strategy compared to previously described techniques for motorized wheel exercise. Unlike voluntary exercise, this allows for controlled experimental induction of running, without the use of aversive stimuli that is typical of treadmill-based techniques.

Conclusions

Adaptive wheel exercise should allow for physical exercise to be better studied as a dynamic, physiological intervention in parkinsonian mice, as well as other neurological disease models.
帕金森病小鼠模型的自适应车轮运动
背景:体育锻炼对神经系统疾病,尤其是帕金森病(PD)的治疗作用已得到广泛研究。然而,现有的小鼠运动技术并不适合运动缺陷的疾病模型动物,这些技术依赖于自发活动或在厌恶刺激下的强制运动,而且不便于通过系留试验对神经生理学进行主动测量。电动轮运动可以克服这些限制,但尚未证明能可靠地诱导小鼠奔跑:新方法:我们开发了一种仪器和技术,可以在没有厌恶刺激的情况下诱导小鼠运动,使用的电动轮可以动态响应受试者的表现:结果:市场上销售的电动轮系统并不能提供令人满意的运动效果,因为小鼠倾向于避免以较高的速度奔跑。我们的自适应车轮运动平台可在6-羟基多巴胺多发性硬化症小鼠模型中进行有效的运动诱导,包括精确的行为测量和同步单体电生理学:与现有方法比较:与之前描述的电动轮运动技术相比,我们的方法提供了一个更好的物理平台和编程策略。与自主运动不同的是,我们的方法可以对跑步进行可控的实验性诱导,而无需使用基于跑步机的技术中常见的厌恶性刺激:结论:自适应车轮运动可以更好地研究体育锻炼对帕金森病小鼠以及其他神经疾病模型的动态生理干预作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Neuroscience Methods
Journal of Neuroscience Methods 医学-神经科学
CiteScore
7.10
自引率
3.30%
发文量
226
审稿时长
52 days
期刊介绍: The Journal of Neuroscience Methods publishes papers that describe new methods that are specifically for neuroscience research conducted in invertebrates, vertebrates or in man. Major methodological improvements or important refinements of established neuroscience methods are also considered for publication. The Journal''s Scope includes all aspects of contemporary neuroscience research, including anatomical, behavioural, biochemical, cellular, computational, molecular, invasive and non-invasive imaging, optogenetic, and physiological research investigations.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信