Origin tracking of Brucella strain B. melitensics bv.3 ARQ-070 using biochemical and genomic studies.

IF 2.2 4区 生物学 Q3 MICROBIOLOGY
Na Ta, Ke-Ming Zuo, Jing Gao, Na Guan, Li-Tao Song, Yong-Jun Wen, Rui-Ping Yu
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引用次数: 0

Abstract

To conduct an origin tracking and genomic study of the Brucella strain B. melitensis bv.3 ARQ-070, with the aim of addressing the challenges posed by the highly conserved genome of Brucella to conventional typing methods and to gain an understanding of the geographic distribution and interspecies transmission of this pathogen in China. Differentiation and genotyping were established via biochemical profiles and polymerase chain reaction. Illumina MiSeq® was applied to sequence the Brucella isolates. Using multilocus sequence typing and Fisher's exact test was used for the KEGG enrichment analysis of differential genes. Differential gene protein-protein interaction (PPI) network analysis was conducted using the STRING database and visualization was performed using. According to the final identification results of an A/M serum agglutination test. ARQ-070 was identified as the No. 3 white spirulina biological variety, and mM was identified as the No. 1 white Spirulina biological variety. The genetic information of this strain is very close to that of the M5 vaccine strain, suggesting possible vaccine-escape infection. In the comparative genomics analysis with B. melitensis bv.1 16 M, the main differences between the B. melitensis strains were found to be concentrated in the genes related to amino acid metabolism and environmental perception. A base mutation was found in the Brucella gene virB9, which is associated with the key virulence factor of the type IV secretion system, but this mutation did not lead to changes in the protein's tertiary structure, and the strain did not lose its infectivity. The study discovered a base mutation in the virB9 gene of Brucella, which is linked to T4SS but does not affect the protein's structure or the strain's infectivity. This mutation could influence public health approaches to detecting and preventing Brucella transmission. Future research aims to analyse a wider range of Brucella strains for a deeper understanding of their epidemiology.

利用生化和基因组研究追踪布鲁氏菌菌株 B. melitensis bv.3 ARQ-070 的起源。
目的对布鲁氏菌B.melitensis bv.3 ARQ-070菌株进行来源追踪和基因组研究,旨在解决布鲁氏菌高度保守的基因组给传统分型方法带来的挑战,并了解该病原体在中国的地理分布和种间传播情况:方法:通过生化图谱和聚合酶链反应(AMOS-PCR)进行分型和基因分型。应用 Illumina MiSeq® 对布鲁氏菌分离株进行测序。使用多焦点序列分型(MLST)多焦点序列分型和费雪精确检验对差异基因进行 KEGG 富集分析。利用 STRING 数据库进行了差异基因蛋白质 PPI 网络分析,并使用了可视化技术:根据 A/M 血清凝集试验的最终鉴定结果。ARQ-070被鉴定为3号白螺旋藻生物品种,mM被鉴定为1号白螺旋藻生物品种。该菌株的遗传信息与 M5 疫苗菌株的遗传信息非常接近,表明可能存在疫苗逃逸感染。在与 B. melitensis bv.1 16 M 的比较基因组学分析中发现,B. melitensis 菌株之间的主要差异集中在与氨基酸代谢和环境感知有关的基因上。在布鲁氏菌基因virB9中发现了一个碱基突变,该基因与IV型分泌系统(T4SS)的关键毒力因子有关,但这一突变并没有导致蛋白质三级结构的改变,菌株也没有失去感染力:该研究发现了布鲁氏菌virB9基因中的一个碱基突变,它与T4SS有关,但不会影响蛋白质的结构或菌株的感染力。这种突变可能会影响检测和预防布鲁氏菌传播的公共卫生方法。未来的研究旨在分析更广泛的布鲁氏菌菌株,以加深对其流行病学的了解。
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来源期刊
Fems Microbiology Letters
Fems Microbiology Letters 生物-微生物学
CiteScore
4.30
自引率
0.00%
发文量
112
审稿时长
1.9 months
期刊介绍: FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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