Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli.

IF 2.2 4区 生物学 Q3 MICROBIOLOGY
Chandrayee Deshamukhya, Ferdous Begom, Bhaskar Jyoti Das, Monjur Ahmed Laskar, Sangeeta Goala, Manabendra Dutta Choudhury, Debadatta Dhar Chanda, Amitabha Bhattacharjee
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引用次数: 0

Abstract

The increasing trend of carbapenem resistance amongst Escherichia coli poses a major public health crisis and requires active surveillance of resistance mechanisms to control the threat. Quorum-sensing system plays a role in bacterial resistance to antibiotics. Quorum sensing is a cell-cell communication system where bacteria alter their gene expression in response to specific stimuli. Here, in this study we investigated the transcriptional response of quorum-sensing receptor, sdiA in E. coli under sub-inhibitory concentration of carbapenem in the presence of quorum-sensing signal molecules. Two E. coli isolates harbouring blaNDM were subjected to treatment with 10% Sodium Dodecyl Sulphate (SDS) for 20 consecutive days of which blaNDM encoding plasmid was successfully eliminated from one isolate. Both the wild type and the cured mutant were then allowed to grow under eight different inducing conditions and the transcriptional response of sdiA gene was studied by quantitative real-time Polymerase Chain Reaction (PCR) methodt. We found different response levels of sdiA in wild type and cured mutant under exogenous AHL and imipenem and when co-cultured with Pseudomonas aeruginosa under imipenem stress. This study highlighted that sub-inhibitory concentration of imipenem in combination with AHL is acting as a signal to SdiA, a quorum-sensing receptor in E. coli.

亚胺培南暴露影响大肠杆菌中法定量感应受体 sdiA 的表达。
大肠埃希菌对碳青霉烯类抗生素的耐药性呈上升趋势,构成了重大的公共卫生危机,需要对耐药性机制进行积极监控,以控制这一威胁。法定人数感应系统在细菌对抗生素的耐药性中发挥着作用。法定人数感应是一种细胞-细胞通信系统,细菌会根据特定刺激改变基因表达。在本研究中,我们研究了大肠杆菌在亚抑制浓度的碳青霉烯类抗生素和法定量感应信号分子作用下,法定量感应受体 sdiA 的转录反应。将两个携带 blaNDM 的大肠杆菌分离株用 10% SDS 连续处理 20 天,其中一个分离株的 blaNDM 编码质粒被成功清除。然后让野生型和固化突变体在 8 种不同的诱导条件下生长,并采用实时定量 PCR 方法研究 sdiA 基因的转录反应。我们发现野生型和固化突变体在外源 AHL 和亚胺培南条件下,以及在亚胺培南胁迫下与铜绿假单胞菌共培养时,sdiA 基因的响应水平不同。这项研究突出表明,亚抑制浓度的亚胺培南与 AHL 联用可作为大肠杆菌中法定量感应受体 SdiA 的信号。
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来源期刊
Fems Microbiology Letters
Fems Microbiology Letters 生物-微生物学
CiteScore
4.30
自引率
0.00%
发文量
112
审稿时长
1.9 months
期刊介绍: FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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