Survival of intact bovine whey proteins across in vivo and simulated static in vitro models of the adult gastrointestinal tract

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-12 DOI:10.1016/j.foodchem.2024.142013

Suwimon Sutantawong, Bum Jin Kim, Yunyao Qu, David C. Dallas

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引用次数: 0

Abstract

Bovine whey contains bioactive proteins that could benefit consumer health, but their intact bioactivity depends on their ability to survive the digestive process and remain intact to their sites of action. Our study assessed whey protein degradation in the adult human jejunum after whey protein isolate ingestion and during static in vitro gastric and intestinal digestions, using LC-MS/MS-based proteomics and SDS-PAGE. We found that 53 % of the protein counts identified in the gastric digesta, including β-lactoglobulin and lactoferrin were stable under simulated gastric conditions. However, most proteins were degraded during both in vitro and in vivo intestinal digestion. The intact protein survival profiles were closely aligned between in vitro and in vivo digestion samples. Understanding the survival of specific whey proteins during digestion will help determine their biological relevancy in their intact forms within the gastrointestinal tract and may inspire strategies to enhance their stability for specific functions.

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完整牛乳清蛋白在成人胃肠道体内模型和模拟静态体外模型中的存活率

牛乳清含有有益于消费者健康的生物活性蛋白质，但其完整的生物活性取决于它们能否在消化过程中存活下来，并完整地留在其作用部位。我们的研究采用基于 LC-MS/MS 的蛋白质组学和 SDS-PAGE 技术，评估了成人在摄入乳清分离蛋白后以及在体外胃肠消化过程中乳清蛋白的降解情况。我们发现，在胃消化物中确定的蛋白质数量中有 53%（包括 β-乳球蛋白和乳铁蛋白）在模拟胃条件下是稳定的。然而，大多数蛋白质在体外和体内肠道消化过程中都会降解。体外消化样本和体内消化样本中完整蛋白质的存活情况非常接近。了解特定乳清蛋白在消化过程中的存活情况将有助于确定它们在胃肠道内的完整生物相关性，并可能激发提高其稳定性以实现特定功能的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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