Efficient one-pot green synthesis of carboxymethyl cellulose/folic acid embedded ultrafine CeO2 nanocomposite and its superior multi-drug resistant antibacterial activity and anticancer activity.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Thalakulam Shanmugam Boopathi, Asha Rajiv, T S Geetika Madan Patel, Lakshay Bareja, Saleh H Salmen, Hossam M Aljawdah, Palanisamy Arulselvan, Jagadeesh Suriyaprakash, Indumathi Thangavelu
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Abstract

Due to the prevalence of drug-resistant bacteria and the ongoing shortage of novel antibiotics as well as the challenge of treating breast cancer, the therapeutic and clinical sectors are consistently seeking effective nanomedicines. The incorporation of metal oxide nanoparticles with biological macromolecules and an organic compound emerges as a promising strategy to enhance breast cancer treatment and antibacterial activity against drug-resistant bacteria in various biomedical applications. This study aims to synthesize a unique nanocomposite consisting of CeO2 embedded with folic acid and carboxymethyl cellulose (CFC NC) via a green precipitation method using Moringa oleifera. Various spectroscopic and microscopic analyses are utilized to decipher the physicochemical characteristics of CFC NC and active phytocompounds of Moringa oleifera. Antibacterial study against MRSA (Methicillin-resistant Staphylococcus aureus) demonstrated a higher activity (95.6%) for CFC NC compared to its counterparts. The impact is attributed to reactive oxygen species (ROS), which induces a strong photo-oxidative stress, leading to the destruction of bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CFC NC are determined as 600 µg/mL and 1000 µg/mL, respectively. The anticancer activity against breast cancer cell resulted in the IC50 concentration of 10.8 μg/mL and 8.2 μg/mL for CeO2 and CFC NC respectively.The biocompatibility test was conducted against fibroblast cells and found 85% of the cells viable, with less toxicity. Therefore, the newly synthesized CFC NC has potential applications in healthcare and industry, enhancing human health conditions.

羧甲基纤维素/叶酸包埋超细CeO2纳米复合材料的高效一锅法绿色合成及其优异的耐多药抗菌活性和抗癌活性。
由于耐药细菌的普遍存在、新型抗生素的持续短缺以及治疗乳腺癌的挑战,治疗和临床部门一直在寻求有效的纳米药物。在各种生物医学应用中,将金属氧化物纳米粒子与生物大分子和有机化合物结合在一起是一种很有前景的策略,可以提高乳腺癌的治疗效果和对耐药细菌的抗菌活性。本研究旨在利用油杉,通过绿色沉淀法合成一种独特的纳米复合材料,由嵌入叶酸和羧甲基纤维素(CFC NC)的 CeO2 组成。利用各种光谱和显微镜分析来解读 CFC NC 和油杉活性植物化合物的理化特性。针对 MRSA(耐甲氧西林金黄色葡萄球菌)的抗菌研究表明,与同类产品相比,CFC NC 的活性更高(95.6%)。这种影响归因于活性氧(ROS),它诱发了强烈的光氧化应激,导致细菌的破坏。CFC NC 的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别为 600 微克/毫升和 1000 微克/毫升。在对乳腺癌细胞的抗癌活性测试中,CeO2 和 CFC NC 的 IC50 浓度分别为 10.8 μg/mL 和 8.2 μg/mL。因此,新合成的 CFC NC 有可能应用于医疗保健和工业领域,改善人类的健康状况。
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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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