Lactobacillus fermentum MCC2760 Attenuates Heated Oil-Induced Brain Oxidative Stress and Inflammation via Modulation of NRF2 and NF-kB in Rats.

Abstract

Scope: Reusing deep-fried oil is a common practice to cut costs, and their consumption may affect brain function. Hence, the study investigates the modulatory potential of Lactobacillus fermentum MCC2760 (LF) on heated oil-induced brain oxidative stress (OS) and inflammation that may have a bearing on cognition in experimental rats.

Methods and results: Female Wistar rats are fed with diets containing native sunflower oil (N-SFO), native canola oil (N-CNO), heated sunflower oil (H-SFO), heated canola oil (H-CNO), heated sunflower oil with probiotic (H-SFO + LF), or heated canola oil with probiotic (H-CNO + LF} for 60 days. Compared to respective controls, heated oils significantly (p < 0.05) increased OS by decreasing antioxidant defense enzymes and nuclear factor erythroid 2-related factor 2 (NRF2) activity. Further, heated oil elevates brain expression of cytosolic phospholipase A2 (cPLA₂), cyclooxygenase-2 (COX-2), prostaglandin-E receptor 4 (EP-4), intercellular adhesion molecule 1 (ICAM-1), nitric oxide synthase 2 (NOS-2), followed by an increased production of proinflammatory eicosanoids (prostaglandin E2 [PGE₂] and leukotriene B4 [LTB₄]) and cytokines (tumor necrosis factor-α [TNF-α], monocyte chemoattractant protein-1 [MCP-1], interleukin-1β [IL-1β], and interleukin-6 [IL-6]). The increased nuclear translocation of nuclear factor kappa beta (NF-kB) in heated oil-fed groups' brains corroborates the heightened inflammatory response. Heated oils decrease neurotrophins and neuron development markers. However, administration of LF abrogates the heated oil-induced alterations significantly (p < 0.05).

Conclusions: The present study is novel in demonstrating the protective role of probiotic LF against heated-oil-induced brain OS and inflammation in rats.