Characterization and antibacterial application of peppermint essential oil nanoemulsions in broiler

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-10-14 DOI:10.1016/j.psj.2024.104432

Muhammad Jabbar , Irfan Baboo , Hamid Majeed , Zahid Farooq , Valiollah Palangi

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引用次数: 0

Abstract

In order to mitigate the risk of antibiotic resistance in poultry, scientists nowadays consider plant secondary metabolites to be a major organic antibacterial substitute. This study aimed to characterize and investigate the in silico, in vitro, and in vivo antibacterial effects of peppermint essential oil (PEO) in the form of a nanoemulsion (NE), termed PEONE. Menthol as a major compound of PEO has been identified by gas chromatography and mass spectroscopy (GCMS) analysis as 32.3 %, while lower droplet size, polydispersity Index (PDI), and optimum zeta potential values depicted the stability of PEONE have been observed and validated by transmission electron microscopy (TEM) micrograph image. In silico antibacterial activity was studied by molecular docking of menthol and enrofloxacin with Topoisomerase IV protein (PDB: 1s16;) of Escherichia coli K12 MG1655 and this effect was validated by in vitro and in vivo analysis. In vitro analysis, sustained release of PEONE has been observed against Escherichia coli and Staphylococcus aureus. In this study for in vivo experiments (n = 90) day-old broiler chicks were distributed into 6 dietary treatments with 5 replicates of 3 birds per replication. Dietary treatments included 1) Negative control (basal diet), 2) Positive control (basal diet + 200 µl enrofloxacin), 3) 25 µl PEONE + basal diet, 4) 50 µl PEONE + basal diet, 5) 75 µl PEONE + basal diet, and 6) 100 µl PEONE + basal diet. Analyzed data by different statistical tools confirmed that PEONE significantly affected body weight gain (BWG) with an improved feed conversion ratio (FCR) compared to the control group. A significant increase in cecal Lactobacillus count and a decrease in total coliform was observed. Positive effects on physiological parameters, visceral organs, and meat quality characteristics have been observed. In conclusion, our experiments suggest that PEONE can be used in the broiler industry as a substitute for antibiotics to minimize bacterial resistance.

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薄荷精油纳米乳剂在肉鸡中的表征和抗菌应用

为了降低家禽对抗生素产生抗药性的风险，如今科学家们认为植物次生代谢物是一种主要的有机抗菌替代品。本研究旨在对纳米乳液（NE）形式的薄荷精油（PEO）（称为 PEONE）的特征、体外和体内抗菌效果进行研究。通过气相色谱和质谱（GCMS）分析，确定薄荷醇是 PEO 的主要化合物，占 32.3%，同时观察到较低的液滴尺寸、多分散指数（PDI）和最佳 zeta 电位值，并通过透射电子显微镜（TEM）显微照片图像验证了 PEONE 的稳定性。通过薄荷醇和恩诺沙星与大肠杆菌 K12 MG1655 的拓扑异构酶 IV 蛋白（PDB：1s16；）的分子对接，对其抗菌活性进行了硅学研究，并通过体外和体内分析验证了这一效果。在体外分析中，观察到 PEONE 对大肠杆菌和金黄色葡萄球菌有持续释放作用。在本研究的体内实验中，一天龄的肉用仔鸡（n = 90）被分为 6 种饮食处理，每种处理有 5 个重复，每个重复 3 只鸡。日粮处理包括：1）阴性对照（基础日粮）；2）阳性对照（基础日粮 + 200 µl 恩诺沙星）；3）25 µl PEONE + 基础日粮；4）50 µl PEONE + 基础日粮；5）75 µl PEONE + 基础日粮；6）100 µl PEONE + 基础日粮。用不同的统计工具分析数据证实，与对照组相比，PEONE 对体重增加（BWG）有明显影响，并提高了饲料转化率（FCR）。粪便中的乳酸杆菌数量明显增加，总大肠菌群减少。还观察到了对生理参数、内脏器官和肉质特征的积极影响。总之，我们的实验表明，PEONE 可作为抗生素的替代品用于肉鸡饲养业，以最大限度地减少细菌耐药性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.