Rapid detection of oil content in Camellia oleifera kernels based on hyperspectral imaging and machine learning

Abstract

The oil content (OC) of kernels is one of the primary targets in the breeding of Camellia oleifera. However, the OC determination is labor-consuming and time-costing using traditional methods. In this study, a rapid and efficient OC detecting method was developed based on hyperspectral imaging (HSI). The OCs of 220 C. oleifera clones were first determined using the Soxtec extraction method and hyperspectral images of all samples were obtained. Five spectral preprocessing methods and two dimensionality reduction methods was performed to eliminate hyperspectral noise. Based on the preprocessed spectral and OC data, OC predictive models were developed. The optimal OC prediction model was developed based on the characteristic wavelengths selected by competitive adaptive reweighted sampling from the preprocessed data by Savitzky–Golay smoothing and the first derivative method. The determination coefficient of this model was 0.9383, with a root mean squared error prediction of 1.7921 % and residual predictive deviation of 4.0271. The further validation of this model by the other samples demonstrated it’s robustness and accuracy. The results reveal the potential of HSI in the rapid OC detection in C. oleifera. This will provide reference and guidance for the phenotype collection of C. oleifera.