Metals in pork lard: Determination by RP DLLME and FAAS, and risk estimation of daily intake

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2024-10-15 DOI:10.1016/j.jfca.2024.106860

Mayara Patricia de Oliveira Santos , Nicoli Andressa Carboni , Eder Lisandro de Moraes Flores , Cristiane Canan , Oldair Donizeti Leite , Deisy Alessandra Drunkler

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引用次数: 0

Abstract

A new method for determining metals (Cu, Fe, Mg, Mn, and Ni) in pork lard was proposed by flame atomic absorption spectrometry (FAAS) using the reversed-phase liquid-liquid dispersive microextraction technique (RP-DLLME) in sample preparation. In optimizing the protocol, the dispersion solvents n-propanol and isopropanol were evaluated, as well as HNO₃ and HNO₃ + HCl (1:1 v v⁻¹) as extracting solvent, the effect of ultrasound and the centrifugation time for the separation of the aqueous extraction phase of the matrix. The estimated limits of quantification for Cu, Fe, Mg, Mn, and Ni were 0.0106, 0.0136, 0.0042, 0.0169, and 0.0124 mg kg⁻¹, respectively. The accuracy for the metals analyzed varied between 95.6 % and 104.4 %, and the precision (RSD) was estimated at around 1 %. Different brands of lard (n = 6) showed recoveries between 90.2 % and 103.2 %, with RSD between 0.024 % and 0.094 %. The estimated daily intake risk for the elements ranged from 0.43 % to 2.79 % for Fe, 0.21–4.22 % for Mg, and 5.13–25.83 % for Ni, of the maximum tolerable intake level. The estimated risk quotient for the metals analyzed was less than 1 ×10⁻⁵, indicating that it is unlikely that sensitive populations will be affected by lard consumption for the metals evaluated.

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猪油中的金属：采用 RP DLLME 和 FAAS 法测定，并对每日摄入量进行风险评估

采用火焰原子吸收光谱法（FAAS）和反相液-液分散微萃取技术（RP-DLLME）制备样品，提出了一种测定猪油中金属（铜、铁、镁、锰和镍）的新方法。在优化方案的过程中，对正丙醇和异丙醇作为分散溶剂、HNO3 和 HNO3 + HCl（1:1 v v-1）作为萃取溶剂、超声波和离心时间对基质水相萃取分离的影响进行了评估。铜、铁、镁、锰和镍的估计定量限分别为 0.0106、0.0136、0.0042、0.0169 和 0.0124 毫克/千克。所分析金属的准确度介于 95.6 % 和 104.4 % 之间，精密度（RSD）约为 1 %。不同品牌的猪油（n = 6）的回收率在 90.2 % 到 103.2 % 之间，RSD 在 0.024 % 到 0.094 % 之间。铁、镁和镍的估计日摄入风险分别为最高耐受摄入量的 0.43 % 至 2.79 %、0.21 % 至 4.22 % 和 5.13 % 至 25.83 %。所分析金属的估计风险商数小于 1 ×10-5，表明敏感人群不太可能因食用猪油而受到所评估金属的影响。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.