Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-10-13 DOI:10.1016/j.psj.2024.104436

Zewei Li , Yifei Chen , Haiping Xie , Quan Li , Shifeng Wang , Huoying Shi

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引用次数: 0

Abstract

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) has resulted in significant losses for the poultry industry worldwide. Currently, there is no widely promoted vaccine for NE. In this study, immunoprecipitation (IP) was employed to isolate immunogenic proteins of C. perfringens, and 118 potential candidate antigens were identified through liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS). From these, three candidate antigen proteins were selected based on their predicted antigenicity, hydrophilicity, stability, and transmembrane signalling properties: ArcB (an ornithine aminotransferase), TmpC (a probable membrane lipoprotein), and EntB (a possible enterotoxin). These three proteins were successfully produced in large quantities using Escherichia coli (E. coli), with confirmed good solubility. Both in vitro and in vivo research demonstrated that these antigens possess strong immunogenicity, eliciting robust antigen-specific humoral and cellular immune responses in chickens and mitigating NE symptoms caused by C. perfringens. The candidate antigens identified through immunoproteomics hold potential as subunit vaccines against C. perfringens infection.

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鉴定免疫原性抗原和评估针对产气荚膜梭菌的候选疫苗。

由产气荚膜梭菌（C. perfringens）引起的坏死性肠炎（NE）给全球家禽业造成了巨大损失。目前，还没有被广泛推广的 NE 疫苗。本研究采用免疫沉淀法（IP）分离了产气荚膜梭菌的免疫原蛋白，并通过液相色谱-质谱/质谱联用技术（LC-MS/MS）鉴定出 118 种潜在的候选抗原。根据预测的抗原性、亲水性、稳定性和跨膜信号特性，从中选出了三种候选抗原蛋白：ArcB（一种鸟氨酸氨基转移酶）、TmpC（一种可能的膜脂蛋白）和 EntB（一种可能的肠毒素）。利用大肠杆菌（E. coli）成功地大量生产了这三种蛋白质，并证实它们具有良好的溶解性。体外和体内研究都表明，这些抗原具有很强的免疫原性，能在鸡体内引起强大的抗原特异性体液和细胞免疫反应，减轻由产气荚膜杆菌引起的 NE 症状。通过免疫蛋白组学鉴定的候选抗原具有作为亚单位疫苗预防C.

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.