Arbutin alleviates Mycoplasma gallinarum-induced damage caused by pulmonary fibrosis via the JAK2/STAT3 pathway

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-10-19 DOI:10.1016/j.psj.2024.104434

Ting Liu , Zhentao Cheng , Derong Song , Erpeng Zhu , Hui Li , Rutao Lin , Zhiling Wan , Shunxing Liu , Zeguang Gong , Chunlan Shan

{"title":"Arbutin alleviates Mycoplasma gallinarum-induced damage caused by pulmonary fibrosis via the JAK2/STAT3 pathway","authors":"Ting Liu , Zhentao Cheng , Derong Song , Erpeng Zhu , Hui Li , Rutao Lin , Zhiling Wan , Shunxing Liu , Zeguang Gong , Chunlan Shan","doi":"10.1016/j.psj.2024.104434","DOIUrl":null,"url":null,"abstract":"<div><div><em>Mycoplasma gallinarum</em> (<em>MG</em>) can cause infectious respiratory diseases in poultry that are chronic. Arbutin (AR) possesses anti-inflammatory, bacteriostatic, antitussive, and expectorant pharmacological effects, but whether it exerts regulatory effects on <em>MG</em>-induced pneumonia and fibrosis remains unclear. The study results unveiled that pulmonary connective tissue hyperplasia, pulmonary capillary congestion, and inflammatory cell infiltration, as well as serum levels of cytokines (i.e., TNF-α, IL-1β, IL-6, and IL-10), were elevated after <em>MG</em> infection. Collagen fibers were significantly deposited in the lung tissue from <em>MG</em>-infected chicks. Furthermore, the expression levels of key factors in the JAK2/STAT3 and TGF-β/Smad pathways markedly increased. AR intervention significantly alleviated <em>MG</em>-induced pneumonic injury, and reduced collagen deposition and the expression of fibrosis markers in the lung tissue. AR reduced the degree of pulmonary fibrosis by regulating key factors of the JAK2/STAT3 signaling pathway in the <em>MG</em>-infected HD11 cells. Thus, AR effectively reduced the expression of inflammatory factors by regulating the JAK2/STAT3 signaling pathway, thereby improving lung inflammation and fibrosis.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"103 12","pages":"Article 104434"},"PeriodicalIF":3.8000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0032579124010125","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Mycoplasma gallinarum (MG) can cause infectious respiratory diseases in poultry that are chronic. Arbutin (AR) possesses anti-inflammatory, bacteriostatic, antitussive, and expectorant pharmacological effects, but whether it exerts regulatory effects on MG-induced pneumonia and fibrosis remains unclear. The study results unveiled that pulmonary connective tissue hyperplasia, pulmonary capillary congestion, and inflammatory cell infiltration, as well as serum levels of cytokines (i.e., TNF-α, IL-1β, IL-6, and IL-10), were elevated after MG infection. Collagen fibers were significantly deposited in the lung tissue from MG-infected chicks. Furthermore, the expression levels of key factors in the JAK2/STAT3 and TGF-β/Smad pathways markedly increased. AR intervention significantly alleviated MG-induced pneumonic injury, and reduced collagen deposition and the expression of fibrosis markers in the lung tissue. AR reduced the degree of pulmonary fibrosis by regulating key factors of the JAK2/STAT3 signaling pathway in the MG-infected HD11 cells. Thus, AR effectively reduced the expression of inflammatory factors by regulating the JAK2/STAT3 signaling pathway, thereby improving lung inflammation and fibrosis.

查看原文本刊更多论文

熊果苷可通过 JAK2/STAT3 通路减轻支原体诱发的肺纤维化损伤。

五倍子支原体（MG）可引起家禽慢性呼吸道传染病。熊果苷（AR）具有抗炎、抑菌、止咳和祛痰等药理作用，但它是否对 MG 诱导的肺炎和纤维化具有调节作用仍不清楚。研究结果显示，MG 感染后肺结缔组织增生、肺毛细血管充血、炎症细胞浸润以及血清细胞因子（即 TNF-α、IL-1β、IL-6 和 IL-10）水平升高。感染 MG 的雏鸡肺组织中胶原纤维明显沉积。此外，JAK2/STAT3 和 TGF-β/Smad 通路中关键因子的表达水平明显升高。AR干预能明显减轻MG诱导的肺损伤，减少肺组织中胶原沉积和纤维化标志物的表达。AR通过调节MG感染的HD11细胞中JAK2/STAT3信号通路的关键因子，减轻了肺纤维化程度。因此，AR通过调节JAK2/STAT3信号通路有效减少了炎症因子的表达，从而改善了肺部炎症和纤维化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.