Molecular Epidemiology of Pseudomonas aeruginosa in Brazil: A Systematic Review and Meta-Analysis.

IF 4.3 2区医学 Q1 INFECTIOUS DISEASES

Antibiotics-Basel Pub Date : 2024-10-17 DOI:10.3390/antibiotics13100983

Yan Corrêa Rodrigues, Marcos Jessé Abrahão Silva, Herald Souza Dos Reis, Pabllo Antonny Silva Dos Santos, Daniele Melo Sardinha, Maria Isabel Montoril Gouveia, Carolynne Silva Dos Santos, Davi Josué Marcon, Caio Augusto Martins Aires, Cintya de Oliveira Souza, Ana Judith Pires Garcia Quaresma, Luana Nepomuceno Gondim Costa Lima, Danielle Murici Brasiliense, Karla Valéria Batista Lima

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引用次数: 0

Abstract

Background: Globally, Pseudomonas aeruginosa is a high-priority opportunistic pathogen which displays several intrinsic and acquired antimicrobial resistance (AMR) mechanisms, leading to challenging treatments and mortality of patients. Moreover, its wide virulence arsenal, particularly the type III secretion system (T3SS) exoU⁺ virulotype, plays a crucial role in pathogenicity and poor outcome of infections. In depth insights into the molecular epidemiology of P. aeruginosa, especially the prevalence of high-risk clones (HRCs), are crucial for the comprehension of virulence and AMR features and their dissemination among distinct strains. This study aims to evaluate the prevalence and distribution of HRCs and non-HRCs among Brazilian isolates of P. aeruginosa.

Methods: A systematic review and meta-analysis were conducted on studies published between 2011 and 2023, focusing on the prevalence of P. aeruginosa clones determined by multilocus sequence typing (MLST) in Brazil. Data were extracted from retrospective cross-sectional and case-control studies, encompassing clinical and non-clinical samples. The analysis included calculating the prevalence rates of various sequence types (STs) and assessing the regional variability in the distribution of HRCs and non-HRCs.

Results: A total of 872 samples were analyzed within all studies, of which 298 (34.17%) were MLST typed, identifying 78 unique STs. HRCs accounted for 48.90% of the MLST-typed isolates, with ST277 being the most prevalent (100/298-33.55%), followed by ST244 (29/298-9.73%), ST235 (13/298-4.36%), ST111 (2/298-0.67%), and ST357 (2/298-0.67%). Significant regional variability was observed, with the Southeast region showing a high prevalence of ST277, while the North region shows a high prevalence of MLST-typed samples and HRCs.

Conclusions: Finally, this systematic review and meta-analysis highlight the role of P. aeruginosa clones in critical issue of AMR in P. aeruginosa in Brazil and the need of integration of comprehensive data from individual studies.

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巴西铜绿假单胞菌的分子流行病学：系统回顾与元分析》。

背景：在全球范围内，铜绿假单胞菌（Pseudomonas aeruginosa）是一种高度优先的机会性病原体，具有多种内在和获得性抗菌药耐药性（AMR）机制，导致治疗困难和患者死亡。此外，其广泛的毒力库，尤其是 III 型分泌系统（T3SS）外显子 U+ 毒力型，在致病性和感染的不良结局中起着至关重要的作用。深入了解铜绿假单胞菌的分子流行病学，特别是高风险克隆（HRCs）的流行情况，对于理解毒力和 AMR 特征及其在不同菌株间的传播至关重要。本研究旨在评估巴西铜绿假单胞菌分离株中高风险克隆和非高风险克隆的流行率和分布情况：方法：对 2011 年至 2023 年间发表的研究进行了系统综述和荟萃分析，重点关注巴西通过多焦点序列分型（MLST）确定的铜绿假单胞菌克隆的流行率。数据提取自回顾性横断面研究和病例对照研究，包括临床和非临床样本。分析包括计算各种序列类型（ST）的患病率，以及评估HRC和非HRC分布的地区差异：结果：所有研究中共分析了 872 份样本，其中 298 份（34.17%）进行了 MLST 分型，确定了 78 种独特的 ST。HRC占MLST分型分离菌株的48.90%，其中ST277最为普遍（100/298-33.55%），其次是ST244（29/298-9.73%）、ST235（13/298-4.36%）、ST111（2/298-0.67%）和ST357（2/298-0.67%）。观察到显著的地区差异，东南部地区 ST277 患病率较高，而北部地区 MLST 类型样本和 HRC 患病率较高：最后，本系统综述和荟萃分析强调了铜绿假单胞菌克隆在巴西铜绿假单胞菌AMR关键问题中的作用，以及整合单项研究综合数据的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.