Genomic Analyses of Methicillin-Resistant Staphylococcus pseudintermedius from Companion Animals Reveal Changing Clonal Populations, Multidrug Resistance, and Virulence.

IF 4.3 2区医学 Q1 INFECTIOUS DISEASES

Antibiotics-Basel Pub Date : 2024-10-12 DOI:10.3390/antibiotics13100962

Mattias Myrenås, Karl Pedersen, Ulrika Windahl

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引用次数: 0

Abstract

Background/objectives: Staphylococcus pseudintermedius is part of the normal microbiota in dogs. Since 2006, an increase in multidrug-resistant clones of methicillin-resistant S. pseudintermedius has been reported, as well as zoonotic transmission. Longitudinal investigations into clonal population structures, antibiotic resistance patterns, and the presence of resistance and virulence genes are important tools for gaining knowledge of the mechanisms behind the emergence of such clones.

Methods: We investigated 87% of all non-repetitive MRSP isolates from dogs and cats in Sweden over a ten-year period (n = 356). All isolates were subjected to staphylococcal chromosomal cassette mec identification, whole-genome sequencing, multi-locus sequence typing, and analyses of genomic relatedness, as well as investigation of phenotypical resistance patterns and the presence of antibiotic resistance genes and virulence genes.

Results: A considerable increase over time in the number of clonal lineages present was observed, indicating genomic diversification, and four clones became dominant: ST71, ST258, ST265, and ST551. In total, 96% of the isolates were multidrug-resistant. Statistically significant differences in resistance to several antibiotic classes between the four dominant clones were present. All isolates carried several virulence genes encoding factors associated with attachment, colonization, toxin synthesis, quorum sensing, antibiotic resistance, and immune evasion.

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对来自伴侣动物的耐甲氧西林假中间葡萄球菌的基因组分析揭示了不断变化的克隆种群、耐多药性和毒性。

背景/目的：伪中间葡萄球菌是狗体内正常微生物群的一部分。自 2006 年以来，耐甲氧西林假中间葡萄球菌的多药耐药克隆以及人畜共患病传播的报道不断增加。对克隆种群结构、抗生素耐药性模式以及耐药性和毒力基因的存在进行纵向调查是了解此类克隆出现背后机制的重要工具：我们调查了十年间瑞典所有猫狗非重复性 MRSP 分离物中的 87%（n = 356）。对所有分离株进行了葡萄球菌染色体盒mec鉴定、全基因组测序、多焦点序列分型和基因组相关性分析，并调查了表型耐药性模式以及抗生素耐药基因和毒力基因的存在情况：结果：观察到随着时间的推移，存在的克隆系数量大幅增加，表明基因组出现了多样化，有四个克隆成为优势克隆：ST71、ST258、ST265 和 ST551。总共有 96% 的分离株具有多重耐药性。据统计，四个优势克隆对几类抗生素的耐药性存在明显差异。所有分离物都携带多种毒力基因，这些基因编码与附着、定殖、毒素合成、法定量感应、抗生素耐药性和免疫逃避相关的因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.