Multi-omics landscape and molecular basis of radiation tolerance in a tardigrade

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2024-10-25 DOI:10.1126/science.adl0799

Lei Li (李磊), Zhengping Ge (葛正平), Shihao Liu (刘世豪), Kun Zheng (郑坤), Yaqi Li (李亚琪), Kaiqi Chen (陈恺骐), Yesheng Fu (付业胜), Xiaoguang Lei (雷晓光), Zeling Cui (崔泽玲), Yifan Wang (王一帆), Jin Huang (黄金), Yanyan Liu (刘艳艳), Mingwang Duan (段明王), Zimei Sun (孙仔妹), Jun Chen (陈俊), Liangwei Li (李良伟), Pan Shen (沈磐), Guibin Wang (王贵宾), Junmiao Chen (陈俊苗), Ruochong Li (李若翀), Chaoran Li (李超然), Zhixiang Yang (杨志向), Yifan Ning (宁一凡), Arong Luo (罗阿蓉), Baoyu Chen (陈宝玉), Inge Seim, Xin Liu (刘心), Fei Wang (王斐), Yishan Yao (姚宜山), Fusheng Guo (郭富生), Maojun Yang (杨茂君), Cui Hua Liu (刘翠华), Guangyi Fan (范广益), Lizhi Wang (王立志), Dong Yang (杨冬), Lingqiang Zhang (张令强)

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Abstract

Tardigrades are captivating organisms known for their resilience in extreme environments, including ultra-high-dose radiation, but the underlying mechanisms of this resilience remain largely unknown. Using genome, transcriptome, and proteome analysis of Hypsibius henanensis sp. nov., we explored the molecular basis contributing to radiotolerance in this organism. A putatively horizontally transferred gene, DOPA dioxygenase 1 (DODA1), responds to radiation and confers radiotolerance by synthesizing betalains—a type of plant pigment with free radical–scavenging properties. A tardigrade-specific radiation-induced disordered protein, TRID1, facilitates DNA damage repair through a mechanism involving phase separation. Two mitochondrial respiratory chain complex assembly proteins, BCS1 and NDUFB8, accumulate to accelerate nicotinamide adenine dinucleotide (NAD⁺) regeneration for poly(adenosine diphosphate–ribosyl)ation (PARylation) and subsequent poly(adenosine diphosphate–ribose) polymerase 1 (PARP1)–mediated DNA damage repair. These three observations expand our understanding of mechanisms of tardigrade radiotolerance.

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沙蜥的多组学特征和耐辐射性的分子基础

迟发型生物以其在极端环境（包括超高剂量辐射）中的恢复能力而闻名，但这种恢复能力的内在机制在很大程度上仍然未知。通过对河南痢疾杆菌（Hypsibius henanensis sp. nov.）的基因组、转录组和蛋白质组分析，我们探索了该生物耐辐射的分子基础。一个被认为是水平转移的基因--DOPA二氧酶1（DODA1）--通过合成甜菜碱--一种具有清除自由基特性的植物色素，对辐射做出反应并赋予辐射耐受性。迟发型特异性辐射诱导紊乱蛋白 TRID1 通过一种涉及相分离的机制促进 DNA 损伤修复。两种线粒体呼吸链复合体组装蛋白--BCS1和NDUFB8--的积累加速了烟酰胺腺嘌呤二核苷酸（NAD+）的再生，从而促进了聚（二磷酸腺苷-核糖）化（PARylation）和随后的聚（二磷酸腺苷-核糖）聚合酶1（PARP1）介导的DNA损伤修复。这三个观察结果拓展了我们对尾蜥放射耐受性机制的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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