Cancer-associated fibroblast-secreted exosomes promote prostate cancer cell migration and invasion by the FGL1/SOX5 axis.

IF 2.5 4区生物学 Q3 CELL BIOLOGY

Histology and histopathology Pub Date : 2024-10-02 DOI:10.14670/HH-18-826

Lingquan Kong, Xing Wang, Yu Li, Xiansheng Zhang

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引用次数: 0

Abstract

Exosomes secreted by cancer-associated fibroblasts (CAFs) play a critical role in cancer progression. This study aimed to explore the effects of CAF exosomes on prostate cancer (PC) cell metastasis. PC cells were treated with these exosomes, and their processes were evaluated using cell-counting kit-8 and Transwell assays. Exosome-regulated mRNAs were explored using quantitative real-time PCR. The relationship between FGL1 and SOX5 was analyzed using co-immunoprecipitation and fluorescence in situ hybridization (FISH) assays. The results of this study showed that exosomes derived from CAFs promoted PC cell viability, migration, and invasion. CAFs promoted PC cell viability and metastasis by releasing exosomes. Exosome treatment increased the levels of FGL1, which interacted with SOX5 and negatively regulated its expression. Rescue experiments demonstrated that CAF exosomes promoted the biological behaviors of PC cells by upregulating FGL1 and downregulating SOX5. Moreover, exosomes accelerated tumor growth by regulating the FGL1 level. In conclusion, CAF-derived exosomes promoted PC cell viability, migration, and invasion by elevating the FGL1/SOX5 axis, suggesting a novel strategy for the treatment of metastatic PC.

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癌症相关成纤维细胞分泌的外泌体通过FGL1/SOX5轴促进前列腺癌细胞迁移和侵袭

癌症相关成纤维细胞（CAF）分泌的外泌体在癌症进展中起着关键作用。本研究旨在探讨CAF外泌体对前列腺癌（PC）细胞转移的影响。用这些外泌体处理 PC 细胞，并用细胞计数试剂盒-8 和 Transwell 试验评估它们的作用过程。利用实时定量 PCR 技术研究了外泌体调控的 mRNA。使用共免疫共沉淀和荧光原位杂交（FISH）方法分析了FGL1和SOX5之间的关系。研究结果表明，来自CAFs的外泌体促进了PC细胞的活力、迁移和侵袭。CAF通过释放外泌体促进了PC细胞的活力和转移。外泌体处理增加了FGL1的水平，FGL1与SOX5相互作用并负向调节其表达。拯救实验表明，CAF外泌体通过上调FGL1和下调SOX5促进了PC细胞的生物学行为。此外，外泌体还通过调节 FGL1 水平加速肿瘤生长。总之，CAF衍生的外泌体通过提高FGL1/SOX5轴促进了PC细胞的活力、迁移和侵袭，为治疗转移性PC提供了一种新策略。

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来源期刊

Histology and histopathology 生物-病理学

CiteScore

3.90

自引率

0.00%

发文量

232

审稿时长

2 months

期刊介绍： HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.