Lower Energy-Demanding Extraction of Bioactive Triterpene Acids by Microwave as the First Step towards Biorefining Residual Olive Skin.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Irene Gómez-Cruz, María Del Mar Contreras, Inmaculada Romero, Eulogio Castro
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Abstract

In the olive oil industry, a pit fraction is collected from olive pomace and split into a clean pit fraction and a residual olive skin-rich fraction, which does not an industrial application. Therefore, in this work, microwave-assisted extraction (MAE) was applied to obtain high-value triterpene acids (maslinic acid and oleanolic acid) from this biomass using the renewable solvent ethanol. The response surface methodology was used to gain a deeper understanding of how the solvent (ethanol-water, 50-100% v/v), time (4-30 min), and temperature (50-120 °C) affect the extraction performance, as well as the energy required for the process. The effect of milling was also studied and the solid-to-liquid ratio was also evaluated, and overall, a good compromise was found at 10% (w/v) using the raw sample (unmilled biomass). The optimised conditions were applied to residual olive skin sourced from various industries, yielding up to 5.1 g/100 g and 2.2 g/100 g dry biomass for maslinic acid and oleanolic acid, respectively. In conclusion, the residual olive skin is a promising natural source of these triterpene acids, which can be extracted using MAE, releasing extracted solids rich in polymeric carbohydrates and lignin that can be valorised under a holistic biorefinery process.

微波提取生物活性三萜酸的能耗较低,这是实现残留橄榄果皮生物精炼的第一步。
在橄榄油工业中,从橄榄果渣中收集果核馏分,并将其分成干净的果核馏分和富含橄榄果皮的残留馏分,但这两种馏分都不能用于工业。因此,本研究采用微波辅助萃取(MAE)技术,利用可再生溶剂乙醇从这种生物质中提取高价值的三萜酸(马斯林酸和齐墩果酸)。该研究采用响应面方法深入了解了溶剂(乙醇-水,50-100% v/v)、时间(4-30 分钟)和温度(50-120 °C)对萃取性能以及过程所需能量的影响。此外,还研究了研磨的影响,并评估了固液比,总体而言,在使用原始样品(未经研磨的生物质)时,10%(w/v)的固液比是一个很好的折中值。将优化条件应用于来自不同行业的残留橄榄果皮,马斯林立酸和齐墩果酸的产量分别高达 5.1 克/100 克和 2.2 克/100 克干生物质。总之,残留的橄榄皮是这些三萜酸的一个很有前景的天然来源,可使用 MAE 进行提取,提取出的固体富含聚合碳水化合物和木质素,可在整体生物精炼工艺中实现价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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