Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Angelo Constantino Camilli, Mariely Araújo de Godoi, Vitória Bonan Costa, Natalie Aparecida Rodrigues Fernandes, Giovani Cirelli, Larissa Kely Faustino da Silva, Letícia Ribeiro Assis, Luis Octavio Regasini, Morgana Rodrigues Guimarães-Stabili
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引用次数: 0

Abstract

Chalcones are phenolic compounds with biological properties. This study had the aim to evaluate the effects of topical administration of a new synthetic chalcone, Chalcone T4, in an animal model of periodontitis induced by ligature. Forty rats were distributed in the following experimental groups: negative control (without periodontitis and topical application of distilled water), positive control (periodontitis and topical application of distilled water), chalcone I and II (periodontitis and topical application of 0.6 mg/mL and 1.8 mg/mL, respectively). Chalcone or distilled water was administered into the gingival sulcus of the first molars daily for 10 days, starting with the ligature installation. The following outcomes were evaluated: alveolar bone loss (µCT and methylene blue dye staining), quantification of osteoclasts (histomorphometry), cell infiltrate and collagen content (stereometry), gene expression of mediators (Nfact11, Tnf-α, Mmp-13, iNos, Sod and Nrf2) by (RT-qPCR); expression of BCL-2 and Caspase-1 (immunohistochemistry). Chalcone T4 inhibited bone resorption and prevented collagen matrix degradation. Reduction in the expression of inflammatory markers (Nfact11, Tnf-α, Mmp-13, and Caspase-1), attenuation of oxidative stress (iNOS reduction, and increase in Sod), and pro-apoptotic effect of the compound (BCL-2 reduction), were associated its effects on periodontal tissues. Topical application of Chalcone T4 prevented bone resorption and inflammation, demonstrating potential in the adjunctive treatment of periodontitis.

局部应用新型查耳酮衍生物(查耳酮 T4)可减轻牙周炎模型大鼠的炎症和氧化应激。
查尔酮是一种具有生物特性的酚类化合物。本研究旨在评估在结扎诱发牙周炎的动物模型中局部施用一种新型合成查尔酮(查尔酮 T4)的效果。40 只大鼠被分为以下实验组:阴性对照组(无牙周炎,外用蒸馏水)、阳性对照组(有牙周炎,外用蒸馏水)、查尔酮 I 和 II 组(有牙周炎,外用剂量分别为 0.6 毫克/毫升和 1.8 毫克/毫升)。从安装结扎器开始,每天在第一磨牙的龈沟中注入查尔酮或蒸馏水,持续 10 天。对以下结果进行了评估:牙槽骨损失(µCT 和亚甲基蓝染色)、破骨细胞的量化(组织形态学)、细胞浸润和胶原蛋白含量(立体测量)、介质(Nfact11、Tnf-α、Mmp-13、iNos、Sod 和 Nrf2)的基因表达(RT-qPCR);BCL-2 和 Caspase-1 的表达(免疫组化)。Chalcone T4 可抑制骨吸收并防止胶原基质降解。减少炎症标志物(Nfact11、Tnf-α、Mmp-13 和 Caspase-1)的表达、减轻氧化应激(iNOS 减少和 Sod 增加)以及促进凋亡效应(BCL-2 减少)与该化合物对牙周组织的影响有关。Chalcone T4 的局部应用可防止骨吸收和炎症,具有辅助治疗牙周炎的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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