In Vitro Screening of Trehalose Synbiotics and Their Effects on Early-Lactating Females and Offspring Mice.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hongmei Peng, Yaya Guo, Jianqiang Zhang, Mengqin Hei, Yuanyuan Li, Wenju Zhang
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Abstract

Activities such as childbirth and breastfeeding can cause severe oxidative stress and inflammatory damage to the mother during early lactation, and can affect animal milk production, and the growth and development of offspring. Trehalose alleviates damage to the body by endowing it with stress resistance. In this study, we used trehalose combined with Lactobacillus plantarum, Bifidobacterium longum, Bacillus subtilis, and Saccharomyces cerevisiae to explore whether dietary intervention can alleviate oxidative stress and inflammatory damage in early lactation and to evaluate the growth ability, acid production ability, antioxidant ability, non-specific adhesion ability, antibacterial ability, and other parameters to determine the optimal combinations and proportions. The results showed that the synbiotics composed of 2.5% trehalose and 1 × 107 cfu/g of Bifidobacterium longum could regulate the gut microbiota, and promote mammary gland development in dams by reducing progesterone (PROG) content in the blood, increasing prolactin (PRL) and insulin-like growth factor-1 (IGF-1) content, enhancing their antioxidant and immune abilities, and effectively increasing the weight and lactation of early lactating dams. In addition, it can also affect the growth of offspring and the development of the intestinal barrier. These results indicate that trehalose synbiotics have great potential in alleviating oxidative stress and inflammatory damage in early lactation.

体外筛选特雷哈尔糖合成益生菌及其对早期泌乳雌性小鼠和后代小鼠的影响
分娩和哺乳等活动会在哺乳早期对母体造成严重的氧化应激和炎症损伤,并影响动物乳汁的分泌和后代的生长发育。曲哈洛糖可通过赋予机体抗应激能力来减轻对机体的损害。在本研究中,我们用曲哈洛糖与植物乳杆菌、长双歧杆菌、枯草芽孢杆菌和酿酒酵母菌结合,探讨膳食干预是否能缓解泌乳早期的氧化应激和炎症损伤,并评估其生长能力、产酸性能、抗氧化能力、非特异性粘附能力、抗菌能力等参数,以确定最佳组合和比例。结果表明,由 2.5% 曲哈糖和 1 × 107 cfu/g 长双歧杆菌组成的益生菌能调节肠道微生物群,通过降低血液中孕酮(PROG)含量、提高催乳素(PRL)和胰岛素样生长因子-1(IGF-1)含量、增强抗氧化能力和免疫能力,促进母畜乳腺发育,有效提高早期泌乳母畜的体重和泌乳量。此外,它还能影响后代的生长和肠道屏障的发育。这些结果表明,三卤糖合生素在缓解泌乳早期氧化应激和炎症损伤方面具有巨大潜力。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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