Evaluation of Glutathione (GSH) System in Porcine Saliva: Validation and Application of Colorimetric Method.

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2024-10-14 DOI:10.3390/antiox13101231

Gamze Gok, Salim Neselioglu, Heng-Lun Ko, Ozcan Erel, María José López-Martínez, Xavier Manteca, Camila Peres Rubio

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引用次数: 0

Abstract

(1) Reduced glutathione (GSH) is considered the first line of antioxidant defense. During oxidative stress, it is oxidized to glutathione disulphide (GSSG). (2) A simple and quick spectrophotometric method based on sodium borohydride (NaBH₄) as a reductant to measure the total and reduced GSH in porcine saliva was analytically validated and evaluated in two situations in this species: (a) in a physiological situation, involving sows during the late lactation and post-weaning periods, and (b) in a situation of sepsis in pigs experimentally induced by LPS administration. (3) The results of the analytical validation showed that the assay was precise and accurate in the porcine saliva samples. Higher total GSH and GSSG and lower reduced GSH were observed in the saliva of sows during the post-weaning period, as well as in pigs with experimentally induced sepsis. (4) In conclusion, the validated assay showed adequate analytical results and could be used to evaluate the GSH system of porcine saliva, as demonstrated during the clinical performance.

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猪唾液中谷胱甘肽（GSH）系统的评估：比色法的验证与应用

(1) 还原型谷胱甘肽（GSH）被认为是抗氧化的第一道防线。在氧化应激过程中，它会被氧化成谷胱甘肽二硫化物（GSSG）。(2) 基于硼氢化钠（NaBH4）作为还原剂的简单快速分光光度法可用于测量猪唾液中的总 GSH 和还原 GSH，该方法在猪的两种情况下进行了分析验证和评估：(a) 哺乳后期和断奶后母猪的生理情况；(b) 注射 LPS 实验诱导猪败血症的情况。(3) 分析验证结果表明，猪唾液样本中的检测结果准确无误。在断奶后母猪和实验性败血症猪的唾液中观察到较高的总 GSH 和 GSSG 以及较低的还原 GSH。(4）总之，验证后的检测方法显示了充分的分析结果，可用于评估猪唾液中的 GSH 系统，这一点在临床表现中也得到了证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.