Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xiaofen Yang, Dongping Zhou, Lv Gao, Yanxin Wang, Yun Wang, Ruru Jia, Yuwei Bai, Deshun Shi, Fenghua Lu
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引用次数: 0

Abstract

The physiological state of Granulosa cells (GCs) is intricately linked to the growth and development of oocytes. Oxidative stress has been found to cause damage to GCs in vitro. Astaxanthin (AST), a well-known natural ketone-type carotenoid, has demonstrated strong antioxidant properties. This study investigates the impact of astaxanthin supplementation on the physiological state of porcine ovarian granulosa cells cultured in vitro. Variations in morphology, apoptosis, reactive oxygen species (ROS) levels, and the expression of apoptosis and anti-oxidation-related genes in porcine GCs from different passages were observed. Significant morphological changes, increases in apoptosis, and decreases in antioxidant capacity resulting from passage were observed. Subsequently, treatment with 5 μmol/L astaxanthin significantly enhanced cell viability, proliferation, antioxidant capacity and mitochondrial function while also regulating the estradiol (E2) and progesterone (P4) levels. Additionally, the gene expression of antioxidation, E2, and P4 synthesis markers was assessed, revealing reduced apoptosis and ROS levels in porcine GCs. In conclusion, supplementation with 5 μmol/L astaxanthin in vitro effectively enhances the physiological condition of porcine GCs and optimizes the culture system for these cells in vitro. Optimizing the culture system of porcine GCs in vitro can simulate the function of granulosa cells in vivo and provide a theoretical reference for further promoting follicular development, which is beneficial to improving sow fertility in actual production.

虾青素对体外培养猪卵巢颗粒细胞生理状态的影响
颗粒细胞(GCs)的生理状态与卵母细胞的生长和发育密切相关。体外研究发现,氧化应激会对颗粒细胞造成损害。虾青素(AST)是一种著名的天然酮类类胡萝卜素,具有很强的抗氧化性。本研究探讨了补充虾青素对体外培养的猪卵巢颗粒细胞生理状态的影响。研究观察了不同传代的猪卵巢颗粒细胞在形态、凋亡、活性氧(ROS)水平以及凋亡和抗氧化相关基因表达方面的变化。观察到不同传代的猪气管细胞形态发生了显著变化,细胞凋亡增加,抗氧化能力下降。随后,用 5 μmol/L 虾青素处理可显著提高细胞活力、增殖、抗氧化能力和线粒体功能,同时还可调节雌二醇(E2)和孕酮(P4)水平。此外,还对抗氧化、E2 和 P4 合成标记的基因表达进行了评估,结果显示猪 GCs 中的细胞凋亡和 ROS 水平有所降低。总之,在体外补充 5 μmol/L 虾青素可有效改善猪 GCs 的生理状况,并优化这些细胞的体外培养系统。优化猪GCs体外培养体系可以模拟体内颗粒细胞的功能,为进一步促进卵泡发育提供理论参考,有利于提高母猪在实际生产中的繁殖力。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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